Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin)
| ISSN: |
0167-4781
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|---|---|
| Keywords: |
(Human fibroblast) ; Alkaline sucrose density gradient centrifugation ; Aphidicolin ; Butylphenyldeoxyguanosine (BuPGdR) ; DNA polymerase ; DNA repair ; Ultraviolet
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| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Medicine
Physics
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798292541020307456 |
|---|---|
| autor | Yamada, K. Itoh, R. |
| autorsonst | Yamada, K. Itoh, R. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0167-4781(94)90052-3 |
| datenlieferant | nat_lic_papers |
| fussnote | DNA polymerases involved in ultraviolet (UV)-induced DNA repair were studied in human fibroblasts using the inhibitors of DNA polymerases, aphidicolin which inhibits DNA polymerases α, δ and ε, and butylphenyldeoxyguanosine (BuPGdR) which inhibits DNA polymerase α strongly and weakly inhibits δ and ε. Both inhibitors inhibited replicative DNA synthesis in a dose dependent manner as measured by thymidine incorporation. However, BuPGdR did not accumulate single strand breaks in cells irradiated with 5 J/m^2 UV-light even at the highest dosage tested, indicating that BuPGdR does not inhibit DNA repair. On the other hand, aphidicolin accumulated single strand breaks in UV-light irradiated cells. These results suggest that DNA polymerase δ and/or ε are mainly involved in UV-induced DNA repair. |
| hauptsatz | hsatz_simple |
| identnr | NLZ186819676 |
| issn | 0167-4781 |
| journal_name | Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 1219 (1994), S. 302-306 |
| schlagwort | (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet |
| search_space | articles |
| shingle_author_1 | Yamada, K. Itoh, R. |
| shingle_author_2 | Yamada, K. Itoh, R. |
| shingle_author_3 | Yamada, K. Itoh, R. |
| shingle_author_4 | Yamada, K. Itoh, R. |
| shingle_catch_all_1 | Yamada, K. Itoh, R. Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet 0167-4781 01674781 Elsevier |
| shingle_catch_all_2 | Yamada, K. Itoh, R. Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet 0167-4781 01674781 Elsevier |
| shingle_catch_all_3 | Yamada, K. Itoh, R. Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet 0167-4781 01674781 Elsevier |
| shingle_catch_all_4 | Yamada, K. Itoh, R. Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet (Human fibroblast) Alkaline sucrose density gradient centrifugation Aphidicolin Butylphenyldeoxyguanosine (BuPGdR) DNA polymerase DNA repair Ultraviolet 0167-4781 01674781 Elsevier |
| shingle_title_1 | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) |
| shingle_title_2 | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) |
| shingle_title_3 | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) |
| shingle_title_4 | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:50:08.941Z |
| titel | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) |
| titel_suche | Involvement of DNA polymerase δ and/or ε in joining UV-induced DNA single strand breaks in human fibroblasts (comparison of effects of butylphenyldeoxyguanosine with aphidicolin) DNA polymerases involved in ultraviolet (UV)-induced DNA repair were studied in human fibroblasts using the inhibitors of DNA polymerases, aphidicolin which inhibits DNA polymerases α, δ and ε, and butylphenyldeoxyguanosine (BuPGdR) which inhibits DNA polymerase α strongly and weakly inhibits δ and ε. Both inhibitors inhibited replicative DNA synthesis in a dose dependent manner as measured by thymidine incorporation. However, BuPGdR did not accumulate single strand breaks in cells irradiated with 5 J/m^2 UV-light even at the highest dosage tested, indicating that BuPGdR does not inhibit DNA repair. On the other hand, aphidicolin accumulated single strand breaks in UV-light irradiated cells. These results suggest that DNA polymerase δ and/or ε are mainly involved in UV-induced DNA repair. |
| topic | W V WW-YZ U |
| uid | nat_lic_papers_NLZ186819676 |