Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus
| ISSN: |
0167-4781
|
|---|---|
| Keywords: |
(Strep. hygroscopicus) ; Chromosome structure ; DNA-binding protein
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
Medicine
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292540585148417 |
|---|---|
| autor | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| autorsonst | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0167-4781(83)90128-8 |
| datenlieferant | nat_lic_papers |
| fussnote | The isolation of the folded chromosomal structure from a Streptomyces strain is described. It is shown that the compact DNA structure of Streptomyces hygroscopicus is stabilized by DNA-RNA interactions. Nucleolytic cleavage decreases the sedimentation rate of the originally isolated (membrane-free) folded Streptomyces chromosome from 1500 S to 800 S or 300 S and finally to completely unfolded DNA. Data on ethidium bromide intercalation suggest a dye-induced relaxation and reintroduction of DNA supertwisting. Like naturally occurring covalently closed circular DNA and like the Escherichia coli chromosome, the folded chromosomal DNA of S. hygroscopicus has about one negative superhelix turn per 200 bp. The results further demonstrate that the isolated Streptomyces nucleoid contains a characteristic S protein which exhibits gel electrophoretic properties of a histone-like component similar to that found in E. coli or Thermoplasma acidophilum. Digestion of the nucleoid with proteinase K at 37^oC causes elimination of the S protein and unfolding of the compact structure. The S protein is also present in other species of the genus Streptomyces. |
| hauptsatz | hsatz_simple |
| identnr | NLZ186815646 |
| issn | 0167-4781 |
| journal_name | Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 740 (1983), S. 118-124 |
| schlagwort | (Strep. hygroscopicus) Chromosome structure DNA-binding protein |
| search_space | articles |
| shingle_author_1 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| shingle_author_2 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| shingle_author_3 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| shingle_author_4 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. |
| shingle_catch_all_1 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus (Strep. hygroscopicus) Chromosome structure DNA-binding protein (Strep. hygroscopicus) Chromosome structure DNA-binding protein 0167-4781 01674781 Elsevier |
| shingle_catch_all_2 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus (Strep. hygroscopicus) Chromosome structure DNA-binding protein (Strep. hygroscopicus) Chromosome structure DNA-binding protein 0167-4781 01674781 Elsevier |
| shingle_catch_all_3 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus (Strep. hygroscopicus) Chromosome structure DNA-binding protein (Strep. hygroscopicus) Chromosome structure DNA-binding protein 0167-4781 01674781 Elsevier |
| shingle_catch_all_4 | Sarfert, E. Zimmer, C. Gumpert, J. Lang, H. Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus (Strep. hygroscopicus) Chromosome structure DNA-binding protein (Strep. hygroscopicus) Chromosome structure DNA-binding protein 0167-4781 01674781 Elsevier |
| shingle_title_1 | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus |
| shingle_title_2 | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus |
| shingle_title_3 | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus |
| shingle_title_4 | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:50:08.941Z |
| titel | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus |
| titel_suche | Folded chromosome structure and DNA-binding protein of Streptomyces hygroscopicus The isolation of the folded chromosomal structure from a Streptomyces strain is described. It is shown that the compact DNA structure of Streptomyces hygroscopicus is stabilized by DNA-RNA interactions. Nucleolytic cleavage decreases the sedimentation rate of the originally isolated (membrane-free) folded Streptomyces chromosome from 1500 S to 800 S or 300 S and finally to completely unfolded DNA. Data on ethidium bromide intercalation suggest a dye-induced relaxation and reintroduction of DNA supertwisting. Like naturally occurring covalently closed circular DNA and like the Escherichia coli chromosome, the folded chromosomal DNA of S. hygroscopicus has about one negative superhelix turn per 200 bp. The results further demonstrate that the isolated Streptomyces nucleoid contains a characteristic S protein which exhibits gel electrophoretic properties of a histone-like component similar to that found in E. coli or Thermoplasma acidophilum. Digestion of the nucleoid with proteinase K at 37^oC causes elimination of the S protein and unfolding of the compact structure. The S protein is also present in other species of the genus Streptomyces. |
| topic | W V WW-YZ U |
| uid | nat_lic_papers_NLZ186815646 |