Roles of antiestrogen binding sites in human endometrial cancer cells
Hayashida, M. ; Terakawa, N. ; Shimizu, I. ; Ikegami, H. ; Wakimoto, H. ; Aono, T. ; Tanizawa, O. ; Matsumoto, K.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0022-4731
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798291106962604032 |
|---|---|
| autor | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| autorsonst | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0022-4731(87)91043-0 |
| datenlieferant | nat_lic_papers |
| fussnote | Estrogen-noncompatible antiestrogen binding sites (AEBS) as well as estrogen receptors (ER), and the growth-inhibitory effect of tamoxifen were investigated in two human endometrial cancer cell lines, IK-90 and HEC-IA cells. IK-90 cells contained specific AEBS, but no ER was found in these cells. Scatchard plot analysis of AEBS in 12,000 g supernatant from IK-90 cells showed a high affinity binding site for tamoxifen (K"d:5.6 +/- 1.0nM) with the maximum binding site of 457 +/- 47 fmol/mg protein. However, no measurable ER or AEBS was found in HEC-IA cells. The effect of tamoxifen on the growth of cells was found to be identical in both cell lines; the addition of 10 μ M tamoxifen to culture medium was cytocidal whereas tamoxifen at lower concentrations (1 nM-1μM) did not significantly affect the growth of both IK-90 and HEC-IA cells. These results demonstrate for the first time the presence of AEBS in human endometrial cancer cells. The present results also suggest that AEBS does not play a fundamental role in mediating the growth:inhibitory effect of tamoxifen in endometrial cancer cells. |
| hauptsatz | hsatz_simple |
| identnr | NLZ18636704X |
| issn | 0022-4731 |
| journal_name | Journal of Steroid Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 26 (1987), S. 705-711 |
| search_space | articles |
| shingle_author_1 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| shingle_author_2 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| shingle_author_3 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| shingle_author_4 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. |
| shingle_catch_all_1 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. Roles of antiestrogen binding sites in human endometrial cancer cells 0022-4731 00224731 Elsevier |
| shingle_catch_all_2 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. Roles of antiestrogen binding sites in human endometrial cancer cells 0022-4731 00224731 Elsevier |
| shingle_catch_all_3 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. Roles of antiestrogen binding sites in human endometrial cancer cells 0022-4731 00224731 Elsevier |
| shingle_catch_all_4 | Hayashida, M. Terakawa, N. Shimizu, I. Ikegami, H. Wakimoto, H. Aono, T. Tanizawa, O. Matsumoto, K. Roles of antiestrogen binding sites in human endometrial cancer cells 0022-4731 00224731 Elsevier |
| shingle_title_1 | Roles of antiestrogen binding sites in human endometrial cancer cells |
| shingle_title_2 | Roles of antiestrogen binding sites in human endometrial cancer cells |
| shingle_title_3 | Roles of antiestrogen binding sites in human endometrial cancer cells |
| shingle_title_4 | Roles of antiestrogen binding sites in human endometrial cancer cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:27:21.447Z |
| titel | Roles of antiestrogen binding sites in human endometrial cancer cells |
| titel_suche | Roles of antiestrogen binding sites in human endometrial cancer cells Estrogen-noncompatible antiestrogen binding sites (AEBS) as well as estrogen receptors (ER), and the growth-inhibitory effect of tamoxifen were investigated in two human endometrial cancer cell lines, IK-90 and HEC-IA cells. IK-90 cells contained specific AEBS, but no ER was found in these cells. Scatchard plot analysis of AEBS in 12,000 g supernatant from IK-90 cells showed a high affinity binding site for tamoxifen (K"d:5.6 +/- 1.0nM) with the maximum binding site of 457 +/- 47 fmol/mg protein. However, no measurable ER or AEBS was found in HEC-IA cells. The effect of tamoxifen on the growth of cells was found to be identical in both cell lines; the addition of 10 μ M tamoxifen to culture medium was cytocidal whereas tamoxifen at lower concentrations (1 nM-1μM) did not significantly affect the growth of both IK-90 and HEC-IA cells. These results demonstrate for the first time the presence of AEBS in human endometrial cancer cells. The present results also suggest that AEBS does not play a fundamental role in mediating the growth:inhibitory effect of tamoxifen in endometrial cancer cells. |
| topic | W V |
| uid | nat_lic_papers_NLZ18636704X |