Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro
| ISSN: |
0005-2760
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Medicine
Physics
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798292218013810689 |
|---|---|
| autor | McCarthy, S. Smith, G.H. |
| autorsonst | McCarthy, S. Smith, G.H. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0005-2760(72)90031-8 |
| datenlieferant | nat_lic_papers |
| fussnote | Slices, homogenates and subcellular fractions of mammary gland tissue from lactating cows and rats were incubated with [1-^1^4C]acetate or dl-β-hydroxy[3-^1^4C]-butyrate and the incorporation of radioactivity into fatty acids was examined.In tissue from the cow, the total incorporation of β-hydroxybutyrate was about one-third of that of acetate, and a larger proportion of the activity incorporated appeared in the shorter-chain fatty acids. The major contribution of β-hydroxybutyrate was as an unbroken C"4 unit at the methyl end of each fatty acid, but a proportion was incorporated throughout the chain after cleavage to C"2 units. Very little incorporation of β-hydroxybutyrate occurred in the isolated ''mitochondrial'' fraction of the cell. Acetate contributed equally to all C"2 units along the length of each fatty acid, except when unlabelled β-hydroxybutyrate was also added to the incubation medium. In this case, the radioactivity in the first two C"2 units (at the methyl end) was diminished. There appeared to be two intracellular sites for the incorporation of acetate: radioactivity was incorporated into short-chain (〈C"1"0) fatty acids in the ''mitochondrial'' fraction, while the longer-chain acids were produced in the ''supernatant'' fraction. The addition of avidin to the incubation medium inhibited the incorporation of radioactivity from β-hydroxybutyrate by homogenates into all acids except C"4; the incorporation of acetate into long-chain acids (〉C"1"0)was also inhibited but the incorporation into short-chain acids was unaffected.A parallel series of experiments with rat tissue allowed a direct comparison with a non-ruminant. In the rat incorporation of both substrates was small and almost entirely confined to the ''supernatant'' fraction. β-Hydroxybutyrate was broken down to C"2 units before incorporation. |
| hauptsatz | hsatz_simple |
| identnr | NLZ185949185 |
| issn | 0005-2760 |
| journal_name | Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 260 (1972), S. 185-196 |
| search_space | articles |
| shingle_author_1 | McCarthy, S. Smith, G.H. |
| shingle_author_2 | McCarthy, S. Smith, G.H. |
| shingle_author_3 | McCarthy, S. Smith, G.H. |
| shingle_author_4 | McCarthy, S. Smith, G.H. |
| shingle_catch_all_1 | McCarthy, S. Smith, G.H. Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro 0005-2760 00052760 Elsevier |
| shingle_catch_all_2 | McCarthy, S. Smith, G.H. Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro 0005-2760 00052760 Elsevier |
| shingle_catch_all_3 | McCarthy, S. Smith, G.H. Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro 0005-2760 00052760 Elsevier |
| shingle_catch_all_4 | McCarthy, S. Smith, G.H. Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro 0005-2760 00052760 Elsevier |
| shingle_title_1 | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro |
| shingle_title_2 | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro |
| shingle_title_3 | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro |
| shingle_title_4 | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:45:01.316Z |
| titel | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro |
| titel_suche | Synthesis of milk fat from β-hydroxybutyrate and acetate by ruminant mammary tissue in vitro Slices, homogenates and subcellular fractions of mammary gland tissue from lactating cows and rats were incubated with [1-^1^4C]acetate or dl-β-hydroxy[3-^1^4C]-butyrate and the incorporation of radioactivity into fatty acids was examined.In tissue from the cow, the total incorporation of β-hydroxybutyrate was about one-third of that of acetate, and a larger proportion of the activity incorporated appeared in the shorter-chain fatty acids. The major contribution of β-hydroxybutyrate was as an unbroken C"4 unit at the methyl end of each fatty acid, but a proportion was incorporated throughout the chain after cleavage to C"2 units. Very little incorporation of β-hydroxybutyrate occurred in the isolated ''mitochondrial'' fraction of the cell. Acetate contributed equally to all C"2 units along the length of each fatty acid, except when unlabelled β-hydroxybutyrate was also added to the incubation medium. In this case, the radioactivity in the first two C"2 units (at the methyl end) was diminished. There appeared to be two intracellular sites for the incorporation of acetate: radioactivity was incorporated into short-chain (〈C"1"0) fatty acids in the ''mitochondrial'' fraction, while the longer-chain acids were produced in the ''supernatant'' fraction. The addition of avidin to the incubation medium inhibited the incorporation of radioactivity from β-hydroxybutyrate by homogenates into all acids except C"4; the incorporation of acetate into long-chain acids (〉C"1"0)was also inhibited but the incorporation into short-chain acids was unaffected.A parallel series of experiments with rat tissue allowed a direct comparison with a non-ruminant. In the rat incorporation of both substrates was small and almost entirely confined to the ''supernatant'' fraction. β-Hydroxybutyrate was broken down to C"2 units before incorporation. |
| topic | W V WW-YZ U |
| uid | nat_lic_papers_NLZ185949185 |