Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements
| ISSN: |
0005-2787
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|---|---|
| Keywords: |
DNA conformation ; DNA damage ; Ultraviolet irradiation
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292379068792832 |
|---|---|
| autor | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| autorsonst | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0005-2787(79)90490-8 |
| datenlieferant | nat_lic_papers |
| fussnote | The paper reports on sedimentation and viscosity measurements performed on ultraviolet-irradiated DNA from T7 phage and calf thymus. From the hydrodynamic data the relative changes in the mean molecular weight, radius of gyration, and effective Kuhn statistical segment length were calculated.The results show that ultraviolet irradiation (254 nm) leads to a significant decrease of the effective statistical segment length of DNA which may be due to small local helix kinks (produced by the generation of photodimers) and a local increase of chain flexibility. Alterations in the overall DNA conformation may be observed even at low fluences where the mean molecular weight almost stays constant. The locally distorted helix regions possibly may serve as recognition sites in the first step of excision repair. |
| hauptsatz | hsatz_simple |
| identnr | NLZ185919073 |
| issn | 0005-2787 |
| journal_name | BBA Section Nucleic Acids And Protein Synthesis |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 561 (1979), S. 59-68 |
| schlagwort | DNA conformation DNA damage Ultraviolet irradiation |
| search_space | articles |
| shingle_author_1 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| shingle_author_2 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| shingle_author_3 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| shingle_author_4 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. |
| shingle_catch_all_1 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements DNA conformation DNA damage Ultraviolet irradiation DNA conformation DNA damage Ultraviolet irradiation 0005-2787 00052787 Elsevier |
| shingle_catch_all_2 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements DNA conformation DNA damage Ultraviolet irradiation DNA conformation DNA damage Ultraviolet irradiation 0005-2787 00052787 Elsevier |
| shingle_catch_all_3 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements DNA conformation DNA damage Ultraviolet irradiation DNA conformation DNA damage Ultraviolet irradiation 0005-2787 00052787 Elsevier |
| shingle_catch_all_4 | Triebel, H. Reinert, K.-E. Bar, H. Lang, H. Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements DNA conformation DNA damage Ultraviolet irradiation DNA conformation DNA damage Ultraviolet irradiation 0005-2787 00052787 Elsevier |
| shingle_title_1 | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements |
| shingle_title_2 | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements |
| shingle_title_3 | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements |
| shingle_title_4 | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:47:35.177Z |
| titel | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements |
| titel_suche | Structural changes of ultraviolet-irradiated DNA derived from hydrodynamic measurements The paper reports on sedimentation and viscosity measurements performed on ultraviolet-irradiated DNA from T7 phage and calf thymus. From the hydrodynamic data the relative changes in the mean molecular weight, radius of gyration, and effective Kuhn statistical segment length were calculated.The results show that ultraviolet irradiation (254 nm) leads to a significant decrease of the effective statistical segment length of DNA which may be due to small local helix kinks (produced by the generation of photodimers) and a local increase of chain flexibility. Alterations in the overall DNA conformation may be observed even at low fluences where the mean molecular weight almost stays constant. The locally distorted helix regions possibly may serve as recognition sites in the first step of excision repair. |
| topic | W |
| uid | nat_lic_papers_NLZ185919073 |