Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells
| ISSN: |
0005-2736
|
|---|---|
| Keywords: |
(Mouse pancreatic β-cell) ; Beta cell membrane ; Cation transport ; Chloride cotransport ; Furosemide ; Rubidium ion efflux ; Sodium ion
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
Medicine
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292201790242816 |
|---|---|
| autor | Sandstrom, P.-E. Sehlin, J. |
| autorsonst | Sandstrom, P.-E. Sehlin, J. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0005-2736(90)90413-I |
| datenlieferant | nat_lic_papers |
| fussnote | In order to investigate whether Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the β-cells, we tested the interaction between the effects of Na^+ deficiency, furosemide and d-glucose on ^8^6Rb^+ fluxes in β-cell-rich mouse pancreatic islets. Removal of extracellular Na^+ slightly reduced the ouabain-resistant ^8^6Rb^+ influx and the specific effect of 1 mM furosemide on this influx was significantly smaller in Na^+-deficient medium. The capacity of 20 mM d-glucose to reduce the ouabain-resistant ^8^6Rb^+ influx was not changed by removal of extracellular Na^+. The ^8^6Rb^+ efflux from preloaded islets was rapidly and reversibly reduced by Na^+ deficiency. Furosemide (1 mM) reduced the ^8^6Rb^+ efflux and the effect of the combination of Na^+ deficiency and 1 mM furosemide was not stronger than the effect of furosemide alone. ^2^2Na^+ efflux was reduced by both ouabain and furosemide and the effects appeared to be additive. The data suggest that Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells. This adds further support to the idea that β-cells exhibit a Na^+,K^+,Cl^- co-transport system. Since some of the furosemide effects on ^8^6Rb^+ efflux persisted in the Na^+-deficient medium, it is likely that also loop diuretic-sensitive K^+,Cl^- co-transport exists in this cell type. |
| hauptsatz | hsatz_simple |
| identnr | NLZ185744400 |
| iqvoc_descriptor_keyword | iqvoc_00000124:transport |
| issn | 0005-2736 |
| journal_name | Biochimica et Biophysica Acta (BBA)/Biomembranes |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 1023 (1990), S. 191-196 |
| schlagwort | (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion |
| search_space | articles |
| shingle_author_1 | Sandstrom, P.-E. Sehlin, J. |
| shingle_author_2 | Sandstrom, P.-E. Sehlin, J. |
| shingle_author_3 | Sandstrom, P.-E. Sehlin, J. |
| shingle_author_4 | Sandstrom, P.-E. Sehlin, J. |
| shingle_catch_all_1 | Sandstrom, P.-E. Sehlin, J. Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion 0005-2736 00052736 Elsevier |
| shingle_catch_all_2 | Sandstrom, P.-E. Sehlin, J. Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion 0005-2736 00052736 Elsevier |
| shingle_catch_all_3 | Sandstrom, P.-E. Sehlin, J. Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion 0005-2736 00052736 Elsevier |
| shingle_catch_all_4 | Sandstrom, P.-E. Sehlin, J. Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion (Mouse pancreatic β-cell) Beta cell membrane Cation transport Chloride cotransport Furosemide Rubidium ion efflux Sodium ion 0005-2736 00052736 Elsevier |
| shingle_title_1 | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells |
| shingle_title_2 | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells |
| shingle_title_3 | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells |
| shingle_title_4 | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:44:45.929Z |
| titel | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells |
| titel_suche | Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells In order to investigate whether Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the β-cells, we tested the interaction between the effects of Na^+ deficiency, furosemide and d-glucose on ^8^6Rb^+ fluxes in β-cell-rich mouse pancreatic islets. Removal of extracellular Na^+ slightly reduced the ouabain-resistant ^8^6Rb^+ influx and the specific effect of 1 mM furosemide on this influx was significantly smaller in Na^+-deficient medium. The capacity of 20 mM d-glucose to reduce the ouabain-resistant ^8^6Rb^+ influx was not changed by removal of extracellular Na^+. The ^8^6Rb^+ efflux from preloaded islets was rapidly and reversibly reduced by Na^+ deficiency. Furosemide (1 mM) reduced the ^8^6Rb^+ efflux and the effect of the combination of Na^+ deficiency and 1 mM furosemide was not stronger than the effect of furosemide alone. ^2^2Na^+ efflux was reduced by both ouabain and furosemide and the effects appeared to be additive. The data suggest that Na^+ participates in loop diuretic-sensitive Cl^--cation co-transport in the pancreatic β-cells. This adds further support to the idea that β-cells exhibit a Na^+,K^+,Cl^- co-transport system. Since some of the furosemide effects on ^8^6Rb^+ efflux persisted in the Na^+-deficient medium, it is likely that also loop diuretic-sensitive K^+,Cl^- co-transport exists in this cell type. |
| topic | W V WW-YZ U |
| uid | nat_lic_papers_NLZ185744400 |