A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis

ISSN:
0304-3991
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Electrical Engineering, Measurement and Control Technology
Natural Sciences in General
Physics
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1016/S0304-3991(81)80196-9
_version_ 1798291810588557312
autor Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
autorsonst Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
book_url http://dx.doi.org/10.1016/S0304-3991(81)80196-9
datenlieferant nat_lic_papers
fussnote A cooling chain is described which enables the transfer of frozen hydrated biological specimens (ultrathin cryosections as well as about 1 μm thick cultured cells) from a cryoultramicrotome into a scanning transmission electron microscope with a field emission gun. Transfer is done at 118 K, specimen temperature in the miscrocope is 165 K. Sublimation processes are controlled visually and by mass spectrometry. Electron micrographs and X-ray microanalytical spectra of cryofixed unstained tissue culture cells and rat liver tissue sections are described and discussed. Contamination of the specimen is much reduced by use of the cold stage.
hauptsatz hsatz_simple
identnr NLZ185251153
issn 0304-3991
journal_name Ultramicroscopy
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 6 (1981), S. 181-186
search_space articles
shingle_author_1 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
shingle_author_2 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
shingle_author_3 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
shingle_author_4 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
shingle_catch_all_1 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
0304-3991
03043991
Elsevier
shingle_catch_all_2 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
0304-3991
03043991
Elsevier
shingle_catch_all_3 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
0304-3991
03043991
Elsevier
shingle_catch_all_4 Zierold, K.
Konig, R.
Olech, K.H.
Schafer, D.
Lubbers, D.W.
Muller, K.H.
Winter, H.
A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
0304-3991
03043991
Elsevier
shingle_title_1 A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
shingle_title_2 A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
shingle_title_3 A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
shingle_title_4 A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
sigel_instance_filter dkfz
geomar
wilbert
ipn
albert
fhp
source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:38:33.101Z
titel A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
titel_suche A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis
A cooling chain is described which enables the transfer of frozen hydrated biological specimens (ultrathin cryosections as well as about 1 μm thick cultured cells) from a cryoultramicrotome into a scanning transmission electron microscope with a field emission gun. Transfer is done at 118 K, specimen temperature in the miscrocope is 165 K. Sublimation processes are controlled visually and by mass spectrometry. Electron micrographs and X-ray microanalytical spectra of cryofixed unstained tissue culture cells and rat liver tissue sections are described and discussed. Contamination of the specimen is much reduced by use of the cold stage.
topic ZN
TA-TD
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uid nat_lic_papers_NLZ185251153