Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells
| ISSN: |
0006-291X
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|---|---|
| Keywords: |
[abr] GDP β S; guanosine 5'-0-2-thiodiphosphate ; [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate ; [abr] GTP; guanosine 5'-triphosphate ; [abr] L current; long-lasting high-threshold Ca^2^+ current ; [abr] T current; transient low-threshold Ca^2^+ current ; [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration
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| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798292260011376640 |
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| autor | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| autorsonst | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| book_url | http://dx.doi.org/10.1016/S0006-291X(05)81526-8 |
| datenlieferant | nat_lic_papers |
| fussnote | We characterized the effects of thyrotropin-releasing hormone (TRH; 500 nM) and guanosine 5'-0-3-thiotriphosphate (GTP γ S; 50 μM) on two types of Ca^2^+ currents in pituitary-hormone-secretory GH"3 cells and were surprised to find marked increases in transient, low-threshold Ca^2^+ currents (T currents) induced by extracellularly applied TRH or intracellularly applied GTP γ S. The effect of TRH was blocked by intracellularly applied guanosine 5'-0-2-thiodiphosphate (GDP βS; 100 μM). The increase in the T current was found to be accompanied by a decrease in long-lasting, high-threshold Ca^2^+ current (L-current), in response to both TRH or GTP γ S. These indicate that the enhancement of Ca^2^+ influx by TRH (500 nM) is largely conferred by T currents in GH"3 cells. A reduced conceptration of TRH (5 nM) still markedly increased the T current, but failed to decrease the L current. These data suggest that the augmentation of the T currents as well as depression of the L currents by TRH (500 nM), through the activation of a GTP-binding protein, may constitute an important regulatory mechanism of sustained pituitary hormone secretion in GH"3 cells. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184999219 |
| issn | 0006-291X |
| journal_name | Biochemical and Biophysical Research Communications |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 187 (1992), S. 529-536 |
| schlagwort | [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration |
| search_space | articles |
| shingle_author_1 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| shingle_author_2 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| shingle_author_3 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| shingle_author_4 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. |
| shingle_catch_all_1 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration 0006-291X 0006291X Elsevier |
| shingle_catch_all_2 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration 0006-291X 0006291X Elsevier |
| shingle_catch_all_3 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration 0006-291X 0006291X Elsevier |
| shingle_catch_all_4 | Suzuki, N. Takagi, H. Yoshioka, T. Tanakadate, A. Kano, M. Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration [abr] GDP β S; guanosine 5'-0-2-thiodiphosphate [abr] GTP γ S; guanosine 5'-0-3-thiotriphosphate [abr] GTP; guanosine 5'-triphosphate [abr] L current; long-lasting high-threshold Ca^2^+ current [abr] T current; transient low-threshold Ca^2^+ current [abr] [Ca^2^+]"1; cytosolic free Ca^2^+ concentration 0006-291X 0006291X Elsevier |
| shingle_title_1 | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells |
| shingle_title_2 | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells |
| shingle_title_3 | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells |
| shingle_title_4 | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:45:40.873Z |
| titel | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells |
| titel_suche | Augmentation of transient low-threshold Ca^2^+ current induced by GTP-binding protein signal transduction system in GH"3 pituitary cells We characterized the effects of thyrotropin-releasing hormone (TRH; 500 nM) and guanosine 5'-0-3-thiotriphosphate (GTP γ S; 50 μM) on two types of Ca^2^+ currents in pituitary-hormone-secretory GH"3 cells and were surprised to find marked increases in transient, low-threshold Ca^2^+ currents (T currents) induced by extracellularly applied TRH or intracellularly applied GTP γ S. The effect of TRH was blocked by intracellularly applied guanosine 5'-0-2-thiodiphosphate (GDP βS; 100 μM). The increase in the T current was found to be accompanied by a decrease in long-lasting, high-threshold Ca^2^+ current (L-current), in response to both TRH or GTP γ S. These indicate that the enhancement of Ca^2^+ influx by TRH (500 nM) is largely conferred by T currents in GH"3 cells. A reduced conceptration of TRH (5 nM) still markedly increased the T current, but failed to decrease the L current. These data suggest that the augmentation of the T currents as well as depression of the L currents by TRH (500 nM), through the activation of a GTP-binding protein, may constitute an important regulatory mechanism of sustained pituitary hormone secretion in GH"3 cells. |
| topic | W V U |
| uid | nat_lic_papers_NLZ184999219 |