Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction
| ISSN: |
1046-5928
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292152847958018 |
|---|---|
| autor | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| autorsonst | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| book_url | http://dx.doi.org/10.1016/1046-5928(91)90070-Y |
| datenlieferant | nat_lic_papers |
| fussnote | We deleted exon 2 in human skeletal β-tropomyosin (hβ-SK tropomyosin) using an improved adaptation of polymerase chain reaction (PCR) technology. The first PCR product was used to prime the full-length cDNA, leading to an exon 2-deleted hβ-SK tropomyosin. This new protein, des-(39-80)-tropomyosin, could then be expressed in Escherichia coli and purified to homogeneity. At the nucleotide level, the junction between exons 1 and 3 has been precisely made in the PCR product. The mutated protein was purified using high-performance liquid chromatography. Des-(39-80)-tropomyosin revealed new immunological properties but was still recognized by certain antitropomyosin antibodies. Furthermore, the structural characteristics of the mutated tropomyosin fit those of the full-length tropomyosin. This new adaptation of PCR technology appears to be suitable for every kind of mutation inside a cloned DNA molecule, and one mutation primer per mutation is sufficient. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184437199 |
| issn | 1046-5928 |
| journal_name | Protein Expression and Purification |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 2 (1991), S. 188-193 |
| search_space | articles |
| shingle_author_1 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| shingle_author_2 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| shingle_author_3 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| shingle_author_4 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. |
| shingle_catch_all_1 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction 1046-5928 10465928 Elsevier |
| shingle_catch_all_2 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction 1046-5928 10465928 Elsevier |
| shingle_catch_all_3 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction 1046-5928 10465928 Elsevier |
| shingle_catch_all_4 | Caron, E. Ferraz, C. Heitz, F. Widada, J.S. Liautard, J.-P. Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction 1046-5928 10465928 Elsevier |
| shingle_title_1 | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction |
| shingle_title_2 | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction |
| shingle_title_3 | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction |
| shingle_title_4 | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:43:58.142Z |
| titel | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction |
| titel_suche | Purification and characterization of an exon 2-deleted human β-tropomyosin constructed by the polymerase chain reaction We deleted exon 2 in human skeletal β-tropomyosin (hβ-SK tropomyosin) using an improved adaptation of polymerase chain reaction (PCR) technology. The first PCR product was used to prime the full-length cDNA, leading to an exon 2-deleted hβ-SK tropomyosin. This new protein, des-(39-80)-tropomyosin, could then be expressed in Escherichia coli and purified to homogeneity. At the nucleotide level, the junction between exons 1 and 3 has been precisely made in the PCR product. The mutated protein was purified using high-performance liquid chromatography. Des-(39-80)-tropomyosin revealed new immunological properties but was still recognized by certain antitropomyosin antibodies. Furthermore, the structural characteristics of the mutated tropomyosin fit those of the full-length tropomyosin. This new adaptation of PCR technology appears to be suitable for every kind of mutation inside a cloned DNA molecule, and one mutation primer per mutation is sufficient. |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ184437199 |