Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells
| ISSN: |
0014-4827
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290808899633152 |
|---|---|
| autor | Rubinstein, N. Chi, J. Holtzer, H. |
| autorsonst | Rubinstein, N. Chi, J. Holtzer, H. |
| book_url | http://dx.doi.org/10.1016/0014-4827(76)90630-3 |
| datenlieferant | nat_lic_papers |
| fussnote | The turnover of myosin and actin in both muscle and non-muscle cells in culture was investigated. By the double-label criterion, myosin and actin were coordinately synthesized and degraded in replicating, mononucleated fibroblasts, chondrocytes, BUdR-suppressed myogenic cells, and in post-mitotic, multinucleated myotubes. Myosin and actin were among the most stable proteins in each cell type. In single label 'pulse-chase' experiments, the half-lives of myosin and actin in all replicating, mononucleated cells were 2.5-3 days; in myotubes, however, they were approx. 6 days. Myosin and actin labelled in replicating presumptive myoblasts and chased until the cells ceased replicating and fused into multinucleated myotubes retained the degradation rate of 3 days; this differed from Jhe rate of 6 days shown for myosin and actin newly-synthesized in post-mitotic myotubes. The type of myosin synthesized in the mother presumptive myoblast, then, is transmitted to the postmitotic daughters. This myosin, however, is more rapidly degraded than the definitive myosin that is synthesized in the myotube. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184369975 |
| issn | 0014-4827 |
| journal_name | Experimental Cell Research |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 97 (1976), S. 387-393 |
| search_space | articles |
| shingle_author_1 | Rubinstein, N. Chi, J. Holtzer, H. |
| shingle_author_2 | Rubinstein, N. Chi, J. Holtzer, H. |
| shingle_author_3 | Rubinstein, N. Chi, J. Holtzer, H. |
| shingle_author_4 | Rubinstein, N. Chi, J. Holtzer, H. |
| shingle_catch_all_1 | Rubinstein, N. Chi, J. Holtzer, H. Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells 0014-4827 00144827 Elsevier |
| shingle_catch_all_2 | Rubinstein, N. Chi, J. Holtzer, H. Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells 0014-4827 00144827 Elsevier |
| shingle_catch_all_3 | Rubinstein, N. Chi, J. Holtzer, H. Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells 0014-4827 00144827 Elsevier |
| shingle_catch_all_4 | Rubinstein, N. Chi, J. Holtzer, H. Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells 0014-4827 00144827 Elsevier |
| shingle_title_1 | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells |
| shingle_title_2 | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells |
| shingle_title_3 | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells |
| shingle_title_4 | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:22:37.684Z |
| titel | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells |
| titel_suche | Coordinated synthesis and degradation of actin and myosin in a variety of myogenic and non-myogenic cells The turnover of myosin and actin in both muscle and non-muscle cells in culture was investigated. By the double-label criterion, myosin and actin were coordinately synthesized and degraded in replicating, mononucleated fibroblasts, chondrocytes, BUdR-suppressed myogenic cells, and in post-mitotic, multinucleated myotubes. Myosin and actin were among the most stable proteins in each cell type. In single label 'pulse-chase' experiments, the half-lives of myosin and actin in all replicating, mononucleated cells were 2.5-3 days; in myotubes, however, they were approx. 6 days. Myosin and actin labelled in replicating presumptive myoblasts and chased until the cells ceased replicating and fused into multinucleated myotubes retained the degradation rate of 3 days; this differed from Jhe rate of 6 days shown for myosin and actin newly-synthesized in post-mitotic myotubes. The type of myosin synthesized in the mother presumptive myoblast, then, is transmitted to the postmitotic daughters. This myosin, however, is more rapidly degraded than the definitive myosin that is synthesized in the myotube. |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ184369975 |