Separation and properties of prestalk and prespore cells of Dictyostelium discoideum
| ISSN: |
0014-4827
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290804293238784 |
|---|---|
| autor | Tsang, A. Bradbury, J.M. |
| autorsonst | Tsang, A. Bradbury, J.M. |
| book_url | http://dx.doi.org/10.1016/0014-4827(81)90118-X |
| datenlieferant | nat_lic_papers |
| fussnote | We describe a method of separating prestalk and prespore cells of Dictyostelium discoideum slugs using a self-generating Percoll gradient. This method gives quantitative recovery of cells and good purity. Separated prestalk and prespore cells possess different levels of the enzymes UDP galactose :polysaccharide transferase, cAMP phosphodiesterase and glycogen phosphorylase. We have used this method, as well as mechanical dissection of slugs, to examine the fate of separated prestalk and prespore cells in Dictyostelium strains that are able to give rise to mature stalk and spore cells in cell monolayers. The results from such experiments provide direct evidence that prestalk and prespore cells from the migrating slug stage are programmed to differentiate into stalk and spore cells respectively. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184330483 |
| issn | 0014-4827 |
| journal_name | Experimental Cell Research |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 132 (1981), S. 433-441 |
| search_space | articles |
| shingle_author_1 | Tsang, A. Bradbury, J.M. |
| shingle_author_2 | Tsang, A. Bradbury, J.M. |
| shingle_author_3 | Tsang, A. Bradbury, J.M. |
| shingle_author_4 | Tsang, A. Bradbury, J.M. |
| shingle_catch_all_1 | Tsang, A. Bradbury, J.M. Separation and properties of prestalk and prespore cells of Dictyostelium discoideum 0014-4827 00144827 Elsevier |
| shingle_catch_all_2 | Tsang, A. Bradbury, J.M. Separation and properties of prestalk and prespore cells of Dictyostelium discoideum 0014-4827 00144827 Elsevier |
| shingle_catch_all_3 | Tsang, A. Bradbury, J.M. Separation and properties of prestalk and prespore cells of Dictyostelium discoideum 0014-4827 00144827 Elsevier |
| shingle_catch_all_4 | Tsang, A. Bradbury, J.M. Separation and properties of prestalk and prespore cells of Dictyostelium discoideum 0014-4827 00144827 Elsevier |
| shingle_title_1 | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum |
| shingle_title_2 | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum |
| shingle_title_3 | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum |
| shingle_title_4 | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:22:33.377Z |
| titel | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum |
| titel_suche | Separation and properties of prestalk and prespore cells of Dictyostelium discoideum We describe a method of separating prestalk and prespore cells of Dictyostelium discoideum slugs using a self-generating Percoll gradient. This method gives quantitative recovery of cells and good purity. Separated prestalk and prespore cells possess different levels of the enzymes UDP galactose :polysaccharide transferase, cAMP phosphodiesterase and glycogen phosphorylase. We have used this method, as well as mechanical dissection of slugs, to examine the fate of separated prestalk and prespore cells in Dictyostelium strains that are able to give rise to mature stalk and spore cells in cell monolayers. The results from such experiments provide direct evidence that prestalk and prespore cells from the migrating slug stage are programmed to differentiate into stalk and spore cells respectively. |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ184330483 |