Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems
Vuorio, A.F. ; Sajantila, A. ; Hamalainen, T. ; Syvanen, A.-C. ; Ehnholm, C. ; Peltonen, L.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0006-291X
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292235894128642 |
|---|---|
| autor | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| autorsonst | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| book_url | http://dx.doi.org/10.1016/0006-291X(90)92136-N |
| datenlieferant | nat_lic_papers |
| fussnote | Our purpose was to identify individuals from blood stains in two murder cases. We used primers flanking the hypervariable region of the apoB gene to amplify DNA extracted from blood stains and blood samples from suspected persons. The sensitivity and specificity of the procedure was improved by carrying out two consecutive PCR amplifications with a nested set of primers in the second amplification. The size of the generated fragments was determined by polyacrylamide gel electrophoresis followed by staining with ethidium bromide. By comparing the fragments produced from the stains with those from the blood samples we were able to identify the origin of the blood stains in both cases. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184082021 |
| issn | 0006-291X |
| journal_name | Biochemical and Biophysical Research Communications |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 170 (1990), S. 616-620 |
| search_space | articles |
| shingle_author_1 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| shingle_author_2 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| shingle_author_3 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| shingle_author_4 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. |
| shingle_catch_all_1 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems 0006-291X 0006291X Elsevier |
| shingle_catch_all_2 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems 0006-291X 0006291X Elsevier |
| shingle_catch_all_3 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems 0006-291X 0006291X Elsevier |
| shingle_catch_all_4 | Vuorio, A.F. Sajantila, A. Hamalainen, T. Syvanen, A.-C. Ehnholm, C. Peltonen, L. Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems 0006-291X 0006291X Elsevier |
| shingle_title_1 | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems |
| shingle_title_2 | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems |
| shingle_title_3 | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems |
| shingle_title_4 | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:45:18.526Z |
| titel | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems |
| titel_suche | Amplification of the hypervariable region close to the apolipoprotein B gene: Application to forensic problems Our purpose was to identify individuals from blood stains in two murder cases. We used primers flanking the hypervariable region of the apoB gene to amplify DNA extracted from blood stains and blood samples from suspected persons. The sensitivity and specificity of the procedure was improved by carrying out two consecutive PCR amplifications with a nested set of primers in the second amplification. The size of the generated fragments was determined by polyacrylamide gel electrophoresis followed by staining with ethidium bromide. By comparing the fragments produced from the stains with those from the blood samples we were able to identify the origin of the blood stains in both cases. |
| topic | W V U |
| uid | nat_lic_papers_NLZ184082021 |