Enzymatic determination of serum-free fatty acids: A colorimetric method
| ISSN: |
0003-2697
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292447164366850 |
|---|---|
| autor | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| autorsonst | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| book_url | http://dx.doi.org/10.1016/0003-2697(80)90511-4 |
| datenlieferant | nat_lic_papers |
| fussnote | A simple and sensitive enzymatic method is described for the determination of serumfree fatty acids. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The reaction is followed as the production of hydrogen peroxide by using acyl-CoA oxidase/peroxidase/4-aminoantipyrine/phenol system. Results on human sera correlate well with those obtained by our previously described enzymatic method (Shimizu et al. (1979) Anal. Biochem. 98, 341-345) and the chemical colorimetric method. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184059925 |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 107 (1980), S. 193-198 |
| search_space | articles |
| shingle_author_1 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| shingle_author_2 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| shingle_author_3 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| shingle_author_4 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. |
| shingle_catch_all_1 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. Enzymatic determination of serum-free fatty acids: A colorimetric method 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. Enzymatic determination of serum-free fatty acids: A colorimetric method 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. Enzymatic determination of serum-free fatty acids: A colorimetric method 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Shimizu, S. Tani, Y. Yamada, H. Tabata, M. Murachi, T. Enzymatic determination of serum-free fatty acids: A colorimetric method 0003-2697 00032697 Elsevier |
| shingle_title_1 | Enzymatic determination of serum-free fatty acids: A colorimetric method |
| shingle_title_2 | Enzymatic determination of serum-free fatty acids: A colorimetric method |
| shingle_title_3 | Enzymatic determination of serum-free fatty acids: A colorimetric method |
| shingle_title_4 | Enzymatic determination of serum-free fatty acids: A colorimetric method |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:39.983Z |
| titel | Enzymatic determination of serum-free fatty acids: A colorimetric method |
| titel_suche | Enzymatic determination of serum-free fatty acids: A colorimetric method A simple and sensitive enzymatic method is described for the determination of serumfree fatty acids. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The reaction is followed as the production of hydrogen peroxide by using acyl-CoA oxidase/peroxidase/4-aminoantipyrine/phenol system. Results on human sera correlate well with those obtained by our previously described enzymatic method (Shimizu et al. (1979) Anal. Biochem. 98, 341-345) and the chemical colorimetric method. |
| topic | W V |
| uid | nat_lic_papers_NLZ184059925 |