Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry
Weng, Q.-M. ; Hammargren, W.M. ; Slowikowski, D. ; Schram, K.H. ; Borysko, K.Z. ; Wotring, L.L. ; Townsend, L.B.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0003-2697
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292445778149376 |
|---|---|
| autor | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| autorsonst | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| book_url | http://dx.doi.org/10.1016/0003-2697(89)90363-1 |
| datenlieferant | nat_lic_papers |
| fussnote | The effect of trimethylsilyl (TMS) derivatization on detection limits of mononucleotides in fast atom bombardment-mass spectrometry (FAB-MS) was examined. FAB-MS methods were developed to optimize sensitivity using adenosine 5'-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5'-monophosphate (TCNMP) and 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP). The detection limit for the TMS derivative of TCNMP was 2.5 - 5 ng/μl and less than 2.5 ng/μl for FdUMP as its TMS derivative. This is greater than two orders of magnitude more sensitive than the FAB-MS analysis of the corresponding free compounds. These low detection limits for the TMS derivatives were obtained using a narrow scan range, signal averaging, detection in the negative ion mode, and 3-nitrobenzyl alcohol as the matrix. Hydrolysis of one or more of the labile TMS groups did occur, with the extent of hydrolysis being greatest in the more protic matrices. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184038448 |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 178 (1989), S. 102-106 |
| search_space | articles |
| shingle_author_1 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| shingle_author_2 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| shingle_author_3 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| shingle_author_4 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. |
| shingle_catch_all_1 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Weng, Q.-M. Hammargren, W.M. Slowikowski, D. Schram, K.H. Borysko, K.Z. Wotring, L.L. Townsend, L.B. Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry 0003-2697 00032697 Elsevier |
| shingle_title_1 | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry |
| shingle_title_2 | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry |
| shingle_title_3 | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry |
| shingle_title_4 | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:38.015Z |
| titel | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry |
| titel_suche | Low nanogram detection of nucleotides using fast atom bombardment-mass spectrometry The effect of trimethylsilyl (TMS) derivatization on detection limits of mononucleotides in fast atom bombardment-mass spectrometry (FAB-MS) was examined. FAB-MS methods were developed to optimize sensitivity using adenosine 5'-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5'-monophosphate (TCNMP) and 5-fluoro-2'-deoxyuridine-5'-monophosphate (FdUMP). The detection limit for the TMS derivative of TCNMP was 2.5 - 5 ng/μl and less than 2.5 ng/μl for FdUMP as its TMS derivative. This is greater than two orders of magnitude more sensitive than the FAB-MS analysis of the corresponding free compounds. These low detection limits for the TMS derivatives were obtained using a narrow scan range, signal averaging, detection in the negative ion mode, and 3-nitrobenzyl alcohol as the matrix. Hydrolysis of one or more of the labile TMS groups did occur, with the extent of hydrolysis being greatest in the more protic matrices. |
| topic | W V |
| uid | nat_lic_papers_NLZ184038448 |