Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation
| ISSN: |
0003-2697
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|---|---|
| Keywords: |
7 S particle ; ammonium sulfate precipitation ; transcription factor IIIA
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| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798292441473744896 |
|---|---|
| autor | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| autorsonst | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| book_url | http://dx.doi.org/10.1016/0003-2697(88)90023-1 |
| datenlieferant | nat_lic_papers |
| fussnote | A simple and efficient method for purifying Xenopus transcription factor IIIA from the 7 S particle has been developed by taking advantage of the differential solubilities of the protein factor and 5 S RNA in ammonium sulfate solution. Conditions under which ammonium sulfate dissociates the 7 S particle and selectively precipitates factor IIIA while the 5 S RNA moiety remains in the supernatant were found. The method simultaneously purifies, in a nondestructive manner, both factor IIIA and 5 S RNA in high yield. Purification proceeds through several ammonium sulfate precipitations of the 7 S particle. Factor IIIA obtained by this method contains no detectable RNA and is highly active as judged by DNase I footprinting and in vitro transcription of the 5 S RNA gene, as well as reconstitution with 5 S RNA to form the 7 S particle. The molar extinction coefficients of factor IIIA at 205 and 280 nm were determined from the ultraviolet absorption spectra measured with the purified protein. |
| hauptsatz | hsatz_simple |
| identnr | NLZ184002486 |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 168 (1988), S. 156-163 |
| schlagwort | 7 S particle ammonium sulfate precipitation transcription factor IIIA |
| search_space | articles |
| shingle_author_1 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| shingle_author_2 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| shingle_author_3 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| shingle_author_4 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. |
| shingle_catch_all_1 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation 7 S particle ammonium sulfate precipitation transcription factor IIIA 7 S particle ammonium sulfate precipitation transcription factor IIIA 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation 7 S particle ammonium sulfate precipitation transcription factor IIIA 7 S particle ammonium sulfate precipitation transcription factor IIIA 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation 7 S particle ammonium sulfate precipitation transcription factor IIIA 7 S particle ammonium sulfate precipitation transcription factor IIIA 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Shang, Z. Windsor, W.T. Liao, Y.-D. Wu, C.-W. Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation 7 S particle ammonium sulfate precipitation transcription factor IIIA 7 S particle ammonium sulfate precipitation transcription factor IIIA 0003-2697 00032697 Elsevier |
| shingle_title_1 | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation |
| shingle_title_2 | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation |
| shingle_title_3 | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation |
| shingle_title_4 | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:34.315Z |
| titel | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation |
| titel_suche | Purification of Xenopus transcription factor IIIA and 5 S RNA from 7 S ribonucleoprotein particle by ammonium sulfate precipitation A simple and efficient method for purifying Xenopus transcription factor IIIA from the 7 S particle has been developed by taking advantage of the differential solubilities of the protein factor and 5 S RNA in ammonium sulfate solution. Conditions under which ammonium sulfate dissociates the 7 S particle and selectively precipitates factor IIIA while the 5 S RNA moiety remains in the supernatant were found. The method simultaneously purifies, in a nondestructive manner, both factor IIIA and 5 S RNA in high yield. Purification proceeds through several ammonium sulfate precipitations of the 7 S particle. Factor IIIA obtained by this method contains no detectable RNA and is highly active as judged by DNase I footprinting and in vitro transcription of the 5 S RNA gene, as well as reconstitution with 5 S RNA to form the 7 S particle. The molar extinction coefficients of factor IIIA at 205 and 280 nm were determined from the ultraviolet absorption spectra measured with the purified protein. |
| topic | W V |
| uid | nat_lic_papers_NLZ184002486 |