Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns
| ISSN: |
0003-2697
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
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| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292438708649985 |
|---|---|
| autor | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| autorsonst | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| book_url | http://dx.doi.org/10.1016/0003-2697(89)90741-0 |
| datenlieferant | nat_lic_papers |
| fussnote | Unwanted reactivity of polyclonal antisera against keratins (''fingerprint proteins'') is a problem commonly encountered when proteins transferred to nitrocellulose are studied by immunoblotting. Immunoreactivity against keratins is generally accompanied by a spotted background. This antikeratin immunoreactivity could be removed by adsorption of the antisera to human keratin bound to nitrocellulose. Larger amounts of antisera were purified from contaminant antikeratin antibodies by a single passage over a column of human keratin coupled to activated CH-Sepharose 4B. In contrast to nonpurified antisera and their IgG fractions, the column effluent no longer recognized the M"r 55,000-70,000 keratin proteins and exhibited a marked decrease in background labeling. We propose this simple method as a valuable alternative when affinity purification of polyclonal antisera on antigen columns is not practical. |
| hauptsatz | hsatz_simple |
| identnr | NLZ18397414X |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 182 (1989), S. 193-196 |
| search_space | articles |
| shingle_author_1 | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| shingle_author_2 | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| shingle_author_3 | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| shingle_author_4 | Girault, J.-A. Gorelick, F.S. Greengard, P. |
| shingle_catch_all_1 | Girault, J.-A. Gorelick, F.S. Greengard, P. Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Girault, J.-A. Gorelick, F.S. Greengard, P. Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Girault, J.-A. Gorelick, F.S. Greengard, P. Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Girault, J.-A. Gorelick, F.S. Greengard, P. Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns 0003-2697 00032697 Elsevier |
| shingle_title_1 | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns |
| shingle_title_2 | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns |
| shingle_title_3 | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns |
| shingle_title_4 | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:31.816Z |
| titel | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns |
| titel_suche | Improving the quality of immunoblots by chromatography of polyclonal antisera on keratin affinity columns Unwanted reactivity of polyclonal antisera against keratins (''fingerprint proteins'') is a problem commonly encountered when proteins transferred to nitrocellulose are studied by immunoblotting. Immunoreactivity against keratins is generally accompanied by a spotted background. This antikeratin immunoreactivity could be removed by adsorption of the antisera to human keratin bound to nitrocellulose. Larger amounts of antisera were purified from contaminant antikeratin antibodies by a single passage over a column of human keratin coupled to activated CH-Sepharose 4B. In contrast to nonpurified antisera and their IgG fractions, the column effluent no longer recognized the M"r 55,000-70,000 keratin proteins and exhibited a marked decrease in background labeling. We propose this simple method as a valuable alternative when affinity purification of polyclonal antisera on antigen columns is not practical. |
| topic | W V |
| uid | nat_lic_papers_NLZ18397414X |