A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters
| ISSN: |
0003-2697
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|---|---|
| Keywords: |
neurochemistry ; nitrocellulose ; protein blotting ; protein kinases ; protein phosphorylation ; synapsin I
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292435540901888 |
|---|---|
| autor | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| autorsonst | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| book_url | http://dx.doi.org/10.1016/0003-2697(86)90600-7 |
| datenlieferant | nat_lic_papers |
| fussnote | A new procedure for the phosphorylation and assay of phosphoproteins is described. Proteins are solubilized from tissue samples, separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane filters, and the blotted polypeptides are phosphorylated with the catalytic subunit of cyclic AMP (adenosine 3':5'-monophosphate)-dependent protein kinase. The method was developed for the assay of dephosphosynapsin I, but it has also proven suitable for the phosphorylation of other proteins. The patterns of phosphorylation of tissue samples phosphorylated using the new method are similar to those obtained using the conventional test tube assay. Once phosphorylated, the adsorbed proteins can be digested with proteases and subjected to phosphopeptide mapping. The phosphorylated blotted proteins can also be analyzed by overlay techniques for the immunological detection of polypeptides. |
| hauptsatz | hsatz_simple |
| identnr | NLZ183948955 |
| issn | 0003-2697 |
| journal_name | Analytical Biochemistry |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 158 (1986), S. 130-137 |
| schlagwort | neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I |
| search_space | articles |
| shingle_author_1 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| shingle_author_2 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| shingle_author_3 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| shingle_author_4 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. |
| shingle_catch_all_1 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I 0003-2697 00032697 Elsevier |
| shingle_catch_all_2 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I 0003-2697 00032697 Elsevier |
| shingle_catch_all_3 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I 0003-2697 00032697 Elsevier |
| shingle_catch_all_4 | Valtorta, F. Schiebler, W. Jahn, R. Ceccarelli, B. Greengard, P. A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I neurochemistry nitrocellulose protein blotting protein kinases protein phosphorylation synapsin I 0003-2697 00032697 Elsevier |
| shingle_title_1 | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters |
| shingle_title_2 | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters |
| shingle_title_3 | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters |
| shingle_title_4 | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:28.953Z |
| titel | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters |
| titel_suche | A solid-phase assay for the phosphorylation of proteins blotted on nitrocellulose membrane filters A new procedure for the phosphorylation and assay of phosphoproteins is described. Proteins are solubilized from tissue samples, separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane filters, and the blotted polypeptides are phosphorylated with the catalytic subunit of cyclic AMP (adenosine 3':5'-monophosphate)-dependent protein kinase. The method was developed for the assay of dephosphosynapsin I, but it has also proven suitable for the phosphorylation of other proteins. The patterns of phosphorylation of tissue samples phosphorylated using the new method are similar to those obtained using the conventional test tube assay. Once phosphorylated, the adsorbed proteins can be digested with proteases and subjected to phosphopeptide mapping. The phosphorylated blotted proteins can also be analyzed by overlay techniques for the immunological detection of polypeptides. |
| topic | W V |
| uid | nat_lic_papers_NLZ183948955 |