Gene-centromere linkage mapping by PCR analysis of individual oocytes

Cui, X. ; Gerwin, J. ; Navidi, W. ; Li, H. ; Kuehn, M. ; Arnheim, N.

Amsterdam : Elsevier
ISSN:
0888-7543
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Biology
Medicine
Type of Medium:
Electronic Resource
URL:
_version_ 1798292084394819584
autor Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
autorsonst Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
book_url http://dx.doi.org/10.1016/0888-7543(92)90146-J
datenlieferant nat_lic_papers
fussnote We describe a general method of determining the recombination fraction between a polymorphic locus and the centromere in any species where single oocytes can be obtained. After removal of the first polar body, each oocyte is analyzed by PCR. The frequency of oocytes heterozygous at the polymorphic locus is used to estimate the recombination fraction. We estimate a recombination fraction of 0.15 between the mouse major histocompatibility complex (H-2) and the centromere of chromosome 17.
hauptsatz hsatz_simple
identnr NLZ183742702
iqvoc_descriptor_title iqvoc_00000708:analysis
issn 0888-7543
journal_name Genomics
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 13 (1992), S. 713-717
search_space articles
shingle_author_1 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
shingle_author_2 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
shingle_author_3 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
shingle_author_4 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
shingle_catch_all_1 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
Gene-centromere linkage mapping by PCR analysis of individual oocytes
0888-7543
08887543
Elsevier
shingle_catch_all_2 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
Gene-centromere linkage mapping by PCR analysis of individual oocytes
0888-7543
08887543
Elsevier
shingle_catch_all_3 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
Gene-centromere linkage mapping by PCR analysis of individual oocytes
0888-7543
08887543
Elsevier
shingle_catch_all_4 Cui, X.
Gerwin, J.
Navidi, W.
Li, H.
Kuehn, M.
Arnheim, N.
Gene-centromere linkage mapping by PCR analysis of individual oocytes
0888-7543
08887543
Elsevier
shingle_title_1 Gene-centromere linkage mapping by PCR analysis of individual oocytes
shingle_title_2 Gene-centromere linkage mapping by PCR analysis of individual oocytes
shingle_title_3 Gene-centromere linkage mapping by PCR analysis of individual oocytes
shingle_title_4 Gene-centromere linkage mapping by PCR analysis of individual oocytes
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source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:42:54.116Z
titel Gene-centromere linkage mapping by PCR analysis of individual oocytes
titel_suche Gene-centromere linkage mapping by PCR analysis of individual oocytes
We describe a general method of determining the recombination fraction between a polymorphic locus and the centromere in any species where single oocytes can be obtained. After removal of the first polar body, each oocyte is analyzed by PCR. The frequency of oocytes heterozygous at the polymorphic locus is used to estimate the recombination fraction. We estimate a recombination fraction of 0.15 between the mouse major histocompatibility complex (H-2) and the centromere of chromosome 17.
topic W
WW-YZ
uid nat_lic_papers_NLZ183742702