The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC
Geraghty, M.T. ; Brody, L.C. ; Martin, L.S. ; Marble, M. ; Kearns, W. ; Pearson, P. ; Monaco, A.P. ; Lehrach, H. ; Valle, D.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0888-7543
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Medicine
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798292082737020928 |
|---|---|
| autor | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| autorsonst | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| book_url | http://dx.doi.org/10.1006/geno.1993.1208 |
| datenlieferant | nat_lic_papers |
| fussnote | Using an ornithine-δ-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 x 10^4 plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of 〉500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region. |
| hauptsatz | hsatz_simple |
| identnr | NLZ183729552 |
| issn | 0888-7543 |
| journal_name | Genomics |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 16 (1993), S. 440-446 |
| search_space | articles |
| shingle_author_1 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| shingle_author_2 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| shingle_author_3 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| shingle_author_4 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. |
| shingle_catch_all_1 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC 0888-7543 08887543 Elsevier |
| shingle_catch_all_2 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC 0888-7543 08887543 Elsevier |
| shingle_catch_all_3 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC 0888-7543 08887543 Elsevier |
| shingle_catch_all_4 | Geraghty, M.T. Brody, L.C. Martin, L.S. Marble, M. Kearns, W. Pearson, P. Monaco, A.P. Lehrach, H. Valle, D. The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC 0888-7543 08887543 Elsevier |
| shingle_title_1 | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC |
| shingle_title_2 | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC |
| shingle_title_3 | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC |
| shingle_title_4 | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:42:52.491Z |
| titel | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC |
| titel_suche | The Isolation of cDNAs from OATL1 at Xp11.2 Using a 480-kb YAC Using an ornithine-δ-aminotransferase (OAT) cDNA, we identified five YACs that cover two nonadjacent OAT-related loci in Xp11.2-p11.3, designated OATL1 (distal) and OATL2 (proximal). Because several retinal degenerative disorders map to this region, we used YAC2 (480 kb), which covers the most distal part of OATL1, as a probe to screen a retinal cDNA library. From 8 x 10^4 plaques screened, we isolated 13 clones. Two were OAT cDNAs. The remaining 11 were divided into eight groups by cross-hybridization. Groups 1-4 contain cDNAs that originate from single-copy X-linked genes in YAC2. Each has an open reading frame of 〉500 bp and detects one or more transcripts on a Northern blot. The gene for each was sublocalized and ordered in YAC2. The cDNAs in groups 5-8 contained two or more Alu sequences, had no open reading frames, and did not detect transcripts. The cDNAs from groups 1-4 provide expressed sequence tags and identify candidate genes for the genetic disorders that map to this region. |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ183729552 |