Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation

ISSN:
0014-4827
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Biology
Medicine
Type of Medium:
Electronic Resource
URL:
_version_ 1798290796774948864
autor Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
autorsonst Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
book_url http://dx.doi.org/10.1016/0014-4827(92)90412-2
datenlieferant nat_lic_papers
fussnote Among the more than 30 different human proteins of the cytokeratin (CK) group of intermediate filament (IF) proteins, the significance of the epidermal polypeptide CK 2 (Moll et al., 1982, Cell 31, 11-24) has been repeatedly questioned in the literature. Here, we show, by in vitro translation and protein gel electrophoresis, that human epidermis from various body sites does indeed contain relatively large amounts of mRNA encoding a distinct polypeptide comigrating with native epidermal CK 2. We also report the isolation of a cDNA clone encoding the complete sequence of CK 2, which is a type II CK different from-but related to-epidermal CKs 1 and 5 on the one hand and corneal CK 3 on the other. The mRNA of ~2.6 kb encodes a polypeptide of 645 amino acids and M"r 65,852, in good agreement with the value of 65.5 kDa previously estimated from gel electrophoresis. This human CK, the largest so far known, displays several features typical of CKs of stratified epithelia, including numerous repeats of glycinerich tetrapeptides in the head and tail domains. Northern blot and in situ hybridizations have shown that CK 2 is expressed strictly suprabasally, usually starting in the third or fourth cell layer of epidermis, and this was confirmed at the protein level by immunohistochemistry using CK 2-specific antibodies. The protein has been detected as a regular epidermal component in skin samples from different body sites, albeit as a minor CK in ''soft skin'' (e.g., breast nipple, penile shaft, axilla), but not in foreskin epithelium and in other epithelia, in squamous metaplasias and carcinomas, or in cultured cell lines derived therefrom. We propose that CK 2 is a late cytoskeletal IF addition synthesized during maturation of epidermal keratinocytes which probably contributes to terminal cornification.
hauptsatz hsatz_simple
identnr NLZ183682092
issn 0014-4827
journal_name Experimental Cell Research
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 202 (1992), S. 132-141
search_space articles
shingle_author_1 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
shingle_author_2 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
shingle_author_3 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
shingle_author_4 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
shingle_catch_all_1 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
0014-4827
00144827
Elsevier
shingle_catch_all_2 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
0014-4827
00144827
Elsevier
shingle_catch_all_3 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
0014-4827
00144827
Elsevier
shingle_catch_all_4 Collin, C.
Moll, R.
Kubicka, S.
Ouhayoun, J.-P.
Franke, W.W.
Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
0014-4827
00144827
Elsevier
shingle_title_1 Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
shingle_title_2 Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
shingle_title_3 Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
shingle_title_4 Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
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source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:22:24.005Z
titel Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
titel_suche Characterization of human cytokeratin 2, an Epidermal cytoskeletal protein synthesized late during differentiation
Among the more than 30 different human proteins of the cytokeratin (CK) group of intermediate filament (IF) proteins, the significance of the epidermal polypeptide CK 2 (Moll et al., 1982, Cell 31, 11-24) has been repeatedly questioned in the literature. Here, we show, by in vitro translation and protein gel electrophoresis, that human epidermis from various body sites does indeed contain relatively large amounts of mRNA encoding a distinct polypeptide comigrating with native epidermal CK 2. We also report the isolation of a cDNA clone encoding the complete sequence of CK 2, which is a type II CK different from-but related to-epidermal CKs 1 and 5 on the one hand and corneal CK 3 on the other. The mRNA of ~2.6 kb encodes a polypeptide of 645 amino acids and M"r 65,852, in good agreement with the value of 65.5 kDa previously estimated from gel electrophoresis. This human CK, the largest so far known, displays several features typical of CKs of stratified epithelia, including numerous repeats of glycinerich tetrapeptides in the head and tail domains. Northern blot and in situ hybridizations have shown that CK 2 is expressed strictly suprabasally, usually starting in the third or fourth cell layer of epidermis, and this was confirmed at the protein level by immunohistochemistry using CK 2-specific antibodies. The protein has been detected as a regular epidermal component in skin samples from different body sites, albeit as a minor CK in ''soft skin'' (e.g., breast nipple, penile shaft, axilla), but not in foreskin epithelium and in other epithelia, in squamous metaplasias and carcinomas, or in cultured cell lines derived therefrom. We propose that CK 2 is a late cytoskeletal IF addition synthesized during maturation of epidermal keratinocytes which probably contributes to terminal cornification.
topic W
WW-YZ
uid nat_lic_papers_NLZ183682092