The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles
| ISSN: |
0006-291X
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292268042420225 |
|---|---|
| autor | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| autorsonst | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| book_url | http://dx.doi.org/10.1016/0006-291X(87)90448-7 |
| datenlieferant | nat_lic_papers |
| fussnote | Although the activating factor F"A of the type-1 protein phosphatase has long been recognized as a cytosolic enzyme involved in the regulation of cell metabolism and nervous functions, strong indications have been obtained that F"A is in fact a membrane-bound protein kinase in most mammalian tissues. For instance, direct treatment of the tissue extracts including brain, liver, cardiac, smooth and skeletal muscles with 1% Triton X-100 can cause several fold stimulation of the F"A activity. Moreover, at least 50% of the F"A can be detected in the particulate fractions of the extracts. Chromatography of the extracts in the presence and absence of Triton X-100 further demonstrate these results. The data can now explain the reason why most people can not isolate reasonable amount of F"A from mammalian tissues. It is recommended that Triton X-100 should be used for purification of F"A from most mammalian tissue extracts. The results also suggest that most previous studies on the action of F"A involved in the regulation of cell functions should be reevaluated and the membrane-associated F"A should be taken into consideration. |
| hauptsatz | hsatz_simple |
| identnr | NLZ183506480 |
| issn | 0006-291X |
| journal_name | Biochemical and Biophysical Research Communications |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 142 (1987), S. 38-46 |
| search_space | articles |
| shingle_author_1 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| shingle_author_2 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| shingle_author_3 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| shingle_author_4 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. |
| shingle_catch_all_1 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles 0006-291X 0006291X Elsevier |
| shingle_catch_all_2 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles 0006-291X 0006291X Elsevier |
| shingle_catch_all_3 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles 0006-291X 0006291X Elsevier |
| shingle_catch_all_4 | Yang, S.-D. Yu, J.-S. Liu, J.-S. Tzen, T.-C. Wang, J.-K. The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles 0006-291X 0006291X Elsevier |
| shingle_title_1 | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles |
| shingle_title_2 | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles |
| shingle_title_3 | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles |
| shingle_title_4 | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:45:48.817Z |
| titel | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles |
| titel_suche | The type-1 protein phosphatase activating factor F"A is a membrane-associated protein kinase in brain, liver, heart and muscles Although the activating factor F"A of the type-1 protein phosphatase has long been recognized as a cytosolic enzyme involved in the regulation of cell metabolism and nervous functions, strong indications have been obtained that F"A is in fact a membrane-bound protein kinase in most mammalian tissues. For instance, direct treatment of the tissue extracts including brain, liver, cardiac, smooth and skeletal muscles with 1% Triton X-100 can cause several fold stimulation of the F"A activity. Moreover, at least 50% of the F"A can be detected in the particulate fractions of the extracts. Chromatography of the extracts in the presence and absence of Triton X-100 further demonstrate these results. The data can now explain the reason why most people can not isolate reasonable amount of F"A from mammalian tissues. It is recommended that Triton X-100 should be used for purification of F"A from most mammalian tissue extracts. The results also suggest that most previous studies on the action of F"A involved in the regulation of cell functions should be reevaluated and the membrane-associated F"A should be taken into consideration. |
| topic | W V U |
| uid | nat_lic_papers_NLZ183506480 |