Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex
Dorbani, L. ; Jancsik, V. ; Linden, M. ; Leterrier, J.F. ; Nelson, B.D. ; Rendon, A.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0003-9861
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
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| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292193764442112 |
|---|---|
| autor | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| autorsonst | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| book_url | http://dx.doi.org/10.1016/0003-9861(87)90023-3 |
| datenlieferant | nat_lic_papers |
| fussnote | Isolated and well-characterized rat brain nonsynaptic mitochondria were subfractionated by digitonin. Antibodies to a uniquely outer membrane protein, porin, have allowed us to use this protein for the first time as an outer membrane marker in brain. Hexokinase, which binds to porin, was also measured. Based upon the sequential release of these and other marker enzymes with increasing concentrations of digitonin, three outer membrane domains have been identified. Two populations of porin were found by this treatment. The most plausible interpretation of our results is that the two porin populations exist in different membrane environments with regard to cholesterol. One of these populations binds most of the hexokinase and appears to be associated with the inner membrane. It is proposed that the porin-hexokinase complex in brain mitochondria is located in a cholesterol-free membrane domain together with inner membrane components. This domain has the features of contact points which have been visualized by electron microscopy. |
| hauptsatz | hsatz_simple |
| identnr | NLZ183416651 |
| issn | 0003-9861 |
| journal_name | Archives of Biochemistry and Biophysics |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 252 (1987), S. 188-196 |
| search_space | articles |
| shingle_author_1 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| shingle_author_2 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| shingle_author_3 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| shingle_author_4 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. |
| shingle_catch_all_1 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex 0003-9861 00039861 Elsevier |
| shingle_catch_all_2 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex 0003-9861 00039861 Elsevier |
| shingle_catch_all_3 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex 0003-9861 00039861 Elsevier |
| shingle_catch_all_4 | Dorbani, L. Jancsik, V. Linden, M. Leterrier, J.F. Nelson, B.D. Rendon, A. Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex 0003-9861 00039861 Elsevier |
| shingle_title_1 | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex |
| shingle_title_2 | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex |
| shingle_title_3 | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex |
| shingle_title_4 | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:44:37.726Z |
| titel | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex |
| titel_suche | Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex Isolated and well-characterized rat brain nonsynaptic mitochondria were subfractionated by digitonin. Antibodies to a uniquely outer membrane protein, porin, have allowed us to use this protein for the first time as an outer membrane marker in brain. Hexokinase, which binds to porin, was also measured. Based upon the sequential release of these and other marker enzymes with increasing concentrations of digitonin, three outer membrane domains have been identified. Two populations of porin were found by this treatment. The most plausible interpretation of our results is that the two porin populations exist in different membrane environments with regard to cholesterol. One of these populations binds most of the hexokinase and appears to be associated with the inner membrane. It is proposed that the porin-hexokinase complex in brain mitochondria is located in a cholesterol-free membrane domain together with inner membrane components. This domain has the features of contact points which have been visualized by electron microscopy. |
| topic | W V U |
| uid | nat_lic_papers_NLZ183416651 |