In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)

Purcell, A.H. ; Steiner, T. ; Megraud, F. ; Bove, J.

Amsterdam : Elsevier
ISSN:
0022-2011
Keywords:
Colladonus ; Euscelidius variegatus ; Euscelus ; Fieberiella ; Macrosteles ; Scaphytopius ; endosymbiont ; symbiote ; transovarial
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Biology
Type of Medium:
Electronic Resource
URL:
_version_ 1798291010379317249
autor Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
autorsonst Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
book_url http://dx.doi.org/10.1016/0022-2011(86)90144-8
datenlieferant nat_lic_papers
fussnote Gram-negative, rod-shaped bacteria were present in the hemolymph of adults and nymphs from colonies of Euscelidius variegatus. the bacterium (designated BEV) was isolated from hemolymph of E. variegatus onto blood (sheep) agar or purple agar with or without added 10% horse or fetal bovine serum. BEV was microaerophilic, catalase- and oxidase-negative, and lacked flagella. Antisera to the cultured bacterium were used in fluorescent antibody staining of BEV-like isolates from E. variegatus or of macerated leafhoppers or smears from leafhoppers. Serological tests indicated that in vitro cultures of BEV were serologically and morphologically identical to the bacterium regularly seen in the hemolymph of E. variegatus from ''infected'' colonies. BEV was detected in and isolated from eggs and nymphs of E. variegatus reared without direct contact with their parents. BEV-free E. variegatus were injected with BEV cultures and the bacterium was reisolated from progeny of the injected insects. BEV appears to be a nonobligate symbiote of E. variegatus. It multiplied and was pathogenic to several other leafhopper species when inoculated by injection.
hauptsatz hsatz_simple
identnr NLZ183133781
issn 0022-2011
journal_name Journal of Invertebrate Pathology
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 48 (1986), S. 66-73
schlagwort Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
search_space articles
shingle_author_1 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
shingle_author_2 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
shingle_author_3 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
shingle_author_4 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
shingle_catch_all_1 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
0022-2011
00222011
Elsevier
shingle_catch_all_2 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
0022-2011
00222011
Elsevier
shingle_catch_all_3 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
0022-2011
00222011
Elsevier
shingle_catch_all_4 Purcell, A.H.
Steiner, T.
Megraud, F.
Bove, J.
In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
Colladonus
Euscelidius variegatus
Euscelus
Fieberiella
Macrosteles
Scaphytopius
endosymbiont
symbiote
transovarial
0022-2011
00222011
Elsevier
shingle_title_1 In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
shingle_title_2 In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
shingle_title_3 In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
shingle_title_4 In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
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source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:25:49.995Z
titel In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
titel_suche In vitro isolation of a transovarially transmitted bacterium from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae)
Gram-negative, rod-shaped bacteria were present in the hemolymph of adults and nymphs from colonies of Euscelidius variegatus. the bacterium (designated BEV) was isolated from hemolymph of E. variegatus onto blood (sheep) agar or purple agar with or without added 10% horse or fetal bovine serum. BEV was microaerophilic, catalase- and oxidase-negative, and lacked flagella. Antisera to the cultured bacterium were used in fluorescent antibody staining of BEV-like isolates from E. variegatus or of macerated leafhoppers or smears from leafhoppers. Serological tests indicated that in vitro cultures of BEV were serologically and morphologically identical to the bacterium regularly seen in the hemolymph of E. variegatus from ''infected'' colonies. BEV was detected in and isolated from eggs and nymphs of E. variegatus reared without direct contact with their parents. BEV-free E. variegatus were injected with BEV cultures and the bacterium was reisolated from progeny of the injected insects. BEV appears to be a nonobligate symbiote of E. variegatus. It multiplied and was pathogenic to several other leafhopper species when inoculated by injection.
topic W
uid nat_lic_papers_NLZ183133781