Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response
| ISSN: |
0011-2240
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|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Medicine
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798292458412441600 |
|---|---|
| autor | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| autorsonst | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| book_url | http://dx.doi.org/10.1016/0011-2240(85)90002-1 |
| datenlieferant | nat_lic_papers |
| fussnote | Human platelets were frozen in 0.5 M glycerol. 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 ^oC/min. The numerical recovery of cells was independent of cooling rate, but freezing altered the cell-size distribution. The combination of dextran and glycerol was no better than glycerol alone at protecting platelets against freezing damage. Mannitol. however, adversely affected platelet 5-HT uptake, and this was reflected in a low recovery of that activity after freezing platelets in glycerol supplemented with mannitol. |
| hauptsatz | hsatz_simple |
| identnr | NLZ183073746 |
| issn | 0011-2240 |
| journal_name | Cryobiology |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 22 (1985), S. 1-9 |
| search_space | articles |
| shingle_author_1 | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| shingle_author_2 | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| shingle_author_3 | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| shingle_author_4 | Brodthagen, U.A. Armitage, W.J. Parmar, N. |
| shingle_catch_all_1 | Brodthagen, U.A. Armitage, W.J. Parmar, N. Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response 0011-2240 00112240 Elsevier |
| shingle_catch_all_2 | Brodthagen, U.A. Armitage, W.J. Parmar, N. Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response 0011-2240 00112240 Elsevier |
| shingle_catch_all_3 | Brodthagen, U.A. Armitage, W.J. Parmar, N. Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response 0011-2240 00112240 Elsevier |
| shingle_catch_all_4 | Brodthagen, U.A. Armitage, W.J. Parmar, N. Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response 0011-2240 00112240 Elsevier |
| shingle_title_1 | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response |
| shingle_title_2 | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response |
| shingle_title_3 | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response |
| shingle_title_4 | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:48:50.620Z |
| titel | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response |
| titel_suche | Platelet cryopreservation with glycerol, dextran, and mannitol: Recovery of 5-hydroxytryptamine uptake and hypotonic stress response Human platelets were frozen in 0.5 M glycerol. 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 ^oC/min. The numerical recovery of cells was independent of cooling rate, but freezing altered the cell-size distribution. The combination of dextran and glycerol was no better than glycerol alone at protecting platelets against freezing damage. Mannitol. however, adversely affected platelet 5-HT uptake, and this was reflected in a low recovery of that activity after freezing platelets in glycerol supplemented with mannitol. |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLZ183073746 |