Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors
| ISSN: |
0022-2836
|
|---|---|
| Keywords: |
arginine repressor ; autoregulation ; repressor-arginine interaction ; repressor-operator interaction
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798291028226080768 |
|---|---|
| autor | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| autorsonst | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| book_url | http://dx.doi.org/10.1016/S0022-2836(05)80028-2 |
| datenlieferant | nat_lic_papers |
| fussnote | In Escherichia coli K12, formation of the enzymes of arginine biosynthesis are controlled by arginine, with complete repression during growth with added arginine, severe repression (about 95%) during growth without added arginine and complete derepression during arginine-limited growth. In E. coli B, the degree of repression is not correlated with arginine concentrations. Under all conditions of growth enzyme formation is repressed, with repression being somewhat less in a medium with arginine than in a medium without arginine. These differences in repressibility between the two strains have been shown previously to be due to the presence of different alleles of argR, the gene for the arginine repressor. Here we have compared the binding of the two repressors to the operator sites of argF (ARG boxes). In DNase I footprinting and gel retardation experiments with argF ARG boxes we have shown that the arginine repressor of E. coli K12 bound to arginine (ArgRK-arg) has a greater affinity than the arginine repressor of E. coli B bound to arginine (ArgRB-arg), whereas free ArgRB (ArgRB"f) has a much stronger affinity than free ArgRK (ArgRK"f). The stronger binding of ArgRB"f can explain the repression seen in E. coli B during arginine-limited growth and indicates that ArgRB"f, but not ArgRK"f, is able to repress enzyme synthesis under physiological conditions. The weaker repression of E. coli B than of E. coli K12 seen in the presence of arginine can be explained by the lower affinity of ArgRB-arg for operator sites as compared to ArgRK-arg. Another contributing cause for the weaker repression is the reduction of ArgRB"f concentration due to autoregulation of the gene for the repressor. Thus the combined effects of repression by ArgRB"f, but not ArgRK"f, with the weaker repression by ArgRB-arg as compared to ArgRK-arg, convert the arginine dependent regulation in E. coli K12 to arginine independent regulation in E. coli B. |
| hauptsatz | hsatz_simple |
| identnr | NLZ177154772 |
| issn | 0022-2836 |
| journal_name | Journal of Molecular Biology |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 235 (1994), S. 221-230 |
| schlagwort | arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction |
| search_space | articles |
| shingle_author_1 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| shingle_author_2 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| shingle_author_3 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| shingle_author_4 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. |
| shingle_catch_all_1 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction 0022-2836 00222836 Elsevier |
| shingle_catch_all_2 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction 0022-2836 00222836 Elsevier |
| shingle_catch_all_3 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction 0022-2836 00222836 Elsevier |
| shingle_catch_all_4 | Tian, G. Lim, D. Oppenheim, J.D. Maas, W.K. Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction arginine repressor autoregulation repressor-arginine interaction repressor-operator interaction 0022-2836 00222836 Elsevier |
| shingle_title_1 | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors |
| shingle_title_2 | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors |
| shingle_title_3 | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors |
| shingle_title_4 | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:26:06.563Z |
| titel | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors |
| titel_suche | Explanation for different types of regulation of arginine biosynthesis in Escherichia coli B and Escherichia coli K12 caused by a difference between their arginine repressors In Escherichia coli K12, formation of the enzymes of arginine biosynthesis are controlled by arginine, with complete repression during growth with added arginine, severe repression (about 95%) during growth without added arginine and complete derepression during arginine-limited growth. In E. coli B, the degree of repression is not correlated with arginine concentrations. Under all conditions of growth enzyme formation is repressed, with repression being somewhat less in a medium with arginine than in a medium without arginine. These differences in repressibility between the two strains have been shown previously to be due to the presence of different alleles of argR, the gene for the arginine repressor. Here we have compared the binding of the two repressors to the operator sites of argF (ARG boxes). In DNase I footprinting and gel retardation experiments with argF ARG boxes we have shown that the arginine repressor of E. coli K12 bound to arginine (ArgRK-arg) has a greater affinity than the arginine repressor of E. coli B bound to arginine (ArgRB-arg), whereas free ArgRB (ArgRB"f) has a much stronger affinity than free ArgRK (ArgRK"f). The stronger binding of ArgRB"f can explain the repression seen in E. coli B during arginine-limited growth and indicates that ArgRB"f, but not ArgRK"f, is able to repress enzyme synthesis under physiological conditions. The weaker repression of E. coli B than of E. coli K12 seen in the presence of arginine can be explained by the lower affinity of ArgRB-arg for operator sites as compared to ArgRK-arg. Another contributing cause for the weaker repression is the reduction of ArgRB"f concentration due to autoregulation of the gene for the repressor. Thus the combined effects of repression by ArgRB"f, but not ArgRK"f, with the weaker repression by ArgRB-arg as compared to ArgRK-arg, convert the arginine dependent regulation in E. coli K12 to arginine independent regulation in E. coli B. |
| topic | W |
| uid | nat_lic_papers_NLZ177154772 |