Separation of DNA restriction fragments by ion-pair chromatography
| ISSN: |
0021-9673
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290931515916288 |
|---|---|
| autor | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| autorsonst | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0021-9673(86)80080-2 |
| datenlieferant | nat_lic_papers |
| fussnote | The separation of restriction endonuclease fragments of DNA on columns of Pharmacia PepRPC (C"2/C"1"8) has been studied. The effect of different concentrations of triethylammonium or tetrabutylammonium salts as ion-pairing reagents, as well as of physical parameters, such as flow-rate and sample load, has been investigated. With the use of triethylammonium buffers, removed by evaporation under vacuum, separated fragments were recovered in yields of 68%. Isolated fragments were accessible to further cleavage with restriction enzymes. Resolution of fragments ranging from 10 to 3000 base pairs depended primarily upon molecular size. |
| hauptsatz | hsatz_simple |
| identnr | NLZ174328184 |
| issn | 0021-9673 |
| journal_name | Journal of Chromatography A |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 359 (1986), S. 265-274 |
| search_space | articles |
| shingle_author_1 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| shingle_author_2 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| shingle_author_3 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| shingle_author_4 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. |
| shingle_catch_all_1 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. Separation of DNA restriction fragments by ion-pair chromatography 0021-9673 00219673 Elsevier |
| shingle_catch_all_2 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. Separation of DNA restriction fragments by ion-pair chromatography 0021-9673 00219673 Elsevier |
| shingle_catch_all_3 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. Separation of DNA restriction fragments by ion-pair chromatography 0021-9673 00219673 Elsevier |
| shingle_catch_all_4 | Eriksson, S. Glad, G. Pernemalm, P.-Å. Westman, E. Separation of DNA restriction fragments by ion-pair chromatography 0021-9673 00219673 Elsevier |
| shingle_title_1 | Separation of DNA restriction fragments by ion-pair chromatography |
| shingle_title_2 | Separation of DNA restriction fragments by ion-pair chromatography |
| shingle_title_3 | Separation of DNA restriction fragments by ion-pair chromatography |
| shingle_title_4 | Separation of DNA restriction fragments by ion-pair chromatography |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:24:34.221Z |
| titel | Separation of DNA restriction fragments by ion-pair chromatography |
| titel_suche | Separation of DNA restriction fragments by ion-pair chromatography The separation of restriction endonuclease fragments of DNA on columns of Pharmacia PepRPC (C"2/C"1"8) has been studied. The effect of different concentrations of triethylammonium or tetrabutylammonium salts as ion-pairing reagents, as well as of physical parameters, such as flow-rate and sample load, has been investigated. With the use of triethylammonium buffers, removed by evaporation under vacuum, separated fragments were recovered in yields of 68%. Isolated fragments were accessible to further cleavage with restriction enzymes. Resolution of fragments ranging from 10 to 3000 base pairs depended primarily upon molecular size. |
| topic | V |
| uid | nat_lic_papers_NLZ174328184 |