Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins
| ISSN: |
0021-9673
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290924019646464 |
|---|---|
| autor | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| autorsonst | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(01)94661-8 |
| datenlieferant | nat_lic_papers |
| fussnote | Many reversed-phase separations of proteins and peptides are currently performed in acidic mobile phases, e.g., 0.1% trifluoroacteic acid in water (pH 2) with organic modifiers. Such conditions are known to promote the cleavage of the silane from the silica in bonded-phase columns, especially for monomeric stationary phases. The stability of some columns commonly used for proteins and peptides has been examined, and it has been shown by both chromatographic and elemental analysis that degradation occurs very rapidly with fresh, ''totally covered'' column materials. Despite the loss of over half of the bonded phase in some cases, certain columns still exhibit adequate chromatographic performance, although reproducibility can be affected. The implications of these results with respect to both bonded-phase synthesis and mechanistic interpretation of chromatographic data is discussed. |
| hauptsatz | hsatz_simple |
| identnr | NLZ174260695 |
| issn | 0021-9673 |
| journal_name | Journal of Chromatography A |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 384 (1987), S. 81-90 |
| search_space | articles |
| shingle_author_1 | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| shingle_author_2 | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| shingle_author_3 | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| shingle_author_4 | Glajch, J.L. Kirkland, J.J. Kohler, J. |
| shingle_catch_all_1 | Glajch, J.L. Kirkland, J.J. Kohler, J. Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins 0021-9673 00219673 Elsevier |
| shingle_catch_all_2 | Glajch, J.L. Kirkland, J.J. Kohler, J. Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins 0021-9673 00219673 Elsevier |
| shingle_catch_all_3 | Glajch, J.L. Kirkland, J.J. Kohler, J. Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins 0021-9673 00219673 Elsevier |
| shingle_catch_all_4 | Glajch, J.L. Kirkland, J.J. Kohler, J. Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins 0021-9673 00219673 Elsevier |
| shingle_title_1 | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins |
| shingle_title_2 | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins |
| shingle_title_3 | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins |
| shingle_title_4 | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:24:27.535Z |
| titel | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins |
| titel_suche | Effect of column degradation on the reversed-phase high-performance liquid chromatographic separation of peptides and proteins Many reversed-phase separations of proteins and peptides are currently performed in acidic mobile phases, e.g., 0.1% trifluoroacteic acid in water (pH 2) with organic modifiers. Such conditions are known to promote the cleavage of the silane from the silica in bonded-phase columns, especially for monomeric stationary phases. The stability of some columns commonly used for proteins and peptides has been examined, and it has been shown by both chromatographic and elemental analysis that degradation occurs very rapidly with fresh, ''totally covered'' column materials. Despite the loss of over half of the bonded phase in some cases, certain columns still exhibit adequate chromatographic performance, although reproducibility can be affected. The implications of these results with respect to both bonded-phase synthesis and mechanistic interpretation of chromatographic data is discussed. |
| topic | V |
| uid | nat_lic_papers_NLZ174260695 |