Factors affecting the ion-pair chromatography of water-soluble vitamins

W. Dong, M. ; Lepore, J. ; Tarumoto, T.

Amsterdam : Elsevier
ISSN:
0021-9673
Source:
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
Topics:
Chemistry and Pharmacology
Type of Medium:
Electronic Resource
URL:
_version_ 1798290915848093697
autor W. Dong, M.
Lepore, J.
Tarumoto, T.
autorsonst W. Dong, M.
Lepore, J.
Tarumoto, T.
book_url http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(00)94458-3
datenlieferant nat_lic_papers
fussnote Factors controlling the separation of seven water-soluble vitamins on reversedphase columnswere systematically evaluated. Factors studied include both mobile phase constituents and column parameters. Data showed that a mobile phase containing hexanesulfonate (5 mM), methanol (15%), acetic acid (1%), and triethylamine (0.10-0.13%) yielded excellent separations with several C"8 and C"1"8 columns. Lowering the methanol concentration in the mobile phase enhanced the resolution of early eluting peaks, while the triethylamine level controlled the peak shape and retention of thiamine. The analytical precision, robustness, and sensitivity of the developedliquid chromatographic (LC) separation were evaluated. The stability of the LC separation was found to be satisfactory for over a 4-month period.
hauptsatz hsatz_simple
identnr NLZ174186711
issn 0021-9673
journal_name Journal of Chromatography A
materialart 1
package_name Elsevier
publikationsort Amsterdam
publisher Elsevier
reference 442 (1988), S. 81-95
search_space articles
shingle_author_1 W. Dong, M.
Lepore, J.
Tarumoto, T.
shingle_author_2 W. Dong, M.
Lepore, J.
Tarumoto, T.
shingle_author_3 W. Dong, M.
Lepore, J.
Tarumoto, T.
shingle_author_4 W. Dong, M.
Lepore, J.
Tarumoto, T.
shingle_catch_all_1 W. Dong, M.
Lepore, J.
Tarumoto, T.
Factors affecting the ion-pair chromatography of water-soluble vitamins
0021-9673
00219673
Elsevier
shingle_catch_all_2 W. Dong, M.
Lepore, J.
Tarumoto, T.
Factors affecting the ion-pair chromatography of water-soluble vitamins
0021-9673
00219673
Elsevier
shingle_catch_all_3 W. Dong, M.
Lepore, J.
Tarumoto, T.
Factors affecting the ion-pair chromatography of water-soluble vitamins
0021-9673
00219673
Elsevier
shingle_catch_all_4 W. Dong, M.
Lepore, J.
Tarumoto, T.
Factors affecting the ion-pair chromatography of water-soluble vitamins
0021-9673
00219673
Elsevier
shingle_title_1 Factors affecting the ion-pair chromatography of water-soluble vitamins
shingle_title_2 Factors affecting the ion-pair chromatography of water-soluble vitamins
shingle_title_3 Factors affecting the ion-pair chromatography of water-soluble vitamins
shingle_title_4 Factors affecting the ion-pair chromatography of water-soluble vitamins
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geomar
wilbert
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albert
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source_archive Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
timestamp 2024-05-06T08:24:19.572Z
titel Factors affecting the ion-pair chromatography of water-soluble vitamins
titel_suche Factors affecting the ion-pair chromatography of water-soluble vitamins
Factors controlling the separation of seven water-soluble vitamins on reversedphase columnswere systematically evaluated. Factors studied include both mobile phase constituents and column parameters. Data showed that a mobile phase containing hexanesulfonate (5 mM), methanol (15%), acetic acid (1%), and triethylamine (0.10-0.13%) yielded excellent separations with several C"8 and C"1"8 columns. Lowering the methanol concentration in the mobile phase enhanced the resolution of early eluting peaks, while the triethylamine level controlled the peak shape and retention of thiamine. The analytical precision, robustness, and sensitivity of the developedliquid chromatographic (LC) separation were evaluated. The stability of the LC separation was found to be satisfactory for over a 4-month period.
topic V
uid nat_lic_papers_NLZ174186711