High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds
| ISSN: |
0021-9673
|
|---|---|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Chemistry and Pharmacology
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290908349726720 |
|---|---|
| autor | Gorham, J. McDonnell, E. |
| autorsonst | Gorham, J. McDonnell, E. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/S0021-9673(01)93523-X |
| datenlieferant | nat_lic_papers |
| fussnote | Procedures are described for the separation and quantitative estimation of choline and glycinebetaine aldehyde in plant saps and extracts. Glycinebetaine aldehyde in the plant material, and a suitable internal standard, were converted to their p-nitrobenzyl oximes during extraction. The extracts was purified on Sep-Pak C"1"8 cartridges and on ion-exchange columns, after which the choline fraction was benzoylated to yield UV-absorbing derivatives. High-performance liquid chromatography was performed on an aliphatic carboxylic acid or an aromatic sulphonic acid cation-exchange column eluted with the phosphate salt of choline in aqueous acetonitrile. The detection limit for both compounds was less than 1 ng. This procedure may also be applied to the analysis of choline in hydrolysates of phospholipids. |
| hauptsatz | hsatz_simple |
| identnr | NLZ174120710 |
| issn | 0021-9673 |
| journal_name | Journal of Chromatography A |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 350 (1985), S. 245-254 |
| search_space | articles |
| shingle_author_1 | Gorham, J. McDonnell, E. |
| shingle_author_2 | Gorham, J. McDonnell, E. |
| shingle_author_3 | Gorham, J. McDonnell, E. |
| shingle_author_4 | Gorham, J. McDonnell, E. |
| shingle_catch_all_1 | Gorham, J. McDonnell, E. High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds 0021-9673 00219673 Elsevier |
| shingle_catch_all_2 | Gorham, J. McDonnell, E. High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds 0021-9673 00219673 Elsevier |
| shingle_catch_all_3 | Gorham, J. McDonnell, E. High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds 0021-9673 00219673 Elsevier |
| shingle_catch_all_4 | Gorham, J. McDonnell, E. High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds 0021-9673 00219673 Elsevier |
| shingle_title_1 | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds |
| shingle_title_2 | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds |
| shingle_title_3 | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds |
| shingle_title_4 | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:24:12.568Z |
| titel | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds |
| titel_suche | High-performance liquid chromatographic method for the separation and estimation of choline, glycinebetaine aldehyde and related compounds Procedures are described for the separation and quantitative estimation of choline and glycinebetaine aldehyde in plant saps and extracts. Glycinebetaine aldehyde in the plant material, and a suitable internal standard, were converted to their p-nitrobenzyl oximes during extraction. The extracts was purified on Sep-Pak C"1"8 cartridges and on ion-exchange columns, after which the choline fraction was benzoylated to yield UV-absorbing derivatives. High-performance liquid chromatography was performed on an aliphatic carboxylic acid or an aromatic sulphonic acid cation-exchange column eluted with the phosphate salt of choline in aqueous acetonitrile. The detection limit for both compounds was less than 1 ng. This procedure may also be applied to the analysis of choline in hydrolysates of phospholipids. |
| topic | V |
| uid | nat_lic_papers_NLZ174120710 |