Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA
| ISSN: |
0014-5793
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|---|---|
| Keywords: |
ATB-BMPA ; Exofacial photolabelling ; Glucose transporter ; Skeletal muscle ; Subcellular fractionation
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290833487691776 |
|---|---|
| autor | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| autorsonst | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)80895-2 |
| datenlieferant | nat_lic_papers |
| fussnote | Employing subcellular membrane fractionation methods it has been shown that insulin induces a 2-fold increase in the Glut 4 protein content in the plasma membrane of skeletal muscle from rats. Data based upon this technique are, however, impeded by poor plasma membrane recovery and cross-contamination with intracellular membrane vesicles. The present study was undertaken to compare the subcellular fractionation technique with the technique using [^3H]ATB-BMPA exofacial photolabelling and immunoprecipitation of Glut 4 on soleus muscles from 3-week-old Wistar rats. Maximal insulin stimulation resulted in a 6-fold increase in 3-O-methylglucose uptake, and studies based on the subcellular fractionation method showed a 2-fold increase in Glut 4 content in the plasma membrane, whereas the exofacial photolabelling demonstrated a 6- to 7-fold rise in cell surface associated Glut 4 protein. Glucose transport activity was positively correlated with cell surface Glut 4 content as estimated by exofacial labelling. In conclusion: (1) the increase in glucose uptake in muscle after insulin exposure is caused by an augmented concentration of Glut 4 protein on the cell surface membrane, (2) at maximal insulin stimulation (20 mUml) approximately 40% of the muscle cell content of Glut 4 is at the cell surface, and (3) the exofacial labelling technique is more sensitive than the subcellular fractionation technique in measuring the amount of glucose transporters on muscle cell surface. |
| hauptsatz | hsatz_simple |
| identnr | NLZ173344356 |
| issn | 0014-5793 |
| journal_name | FEBS Letters |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 330 (1993), S. 312-318 |
| schlagwort | ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation |
| search_space | articles |
| shingle_author_1 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| shingle_author_2 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| shingle_author_3 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| shingle_author_4 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. |
| shingle_catch_all_1 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation 0014-5793 00145793 Elsevier |
| shingle_catch_all_2 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation 0014-5793 00145793 Elsevier |
| shingle_catch_all_3 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation 0014-5793 00145793 Elsevier |
| shingle_catch_all_4 | Lund, S. Holman, G.D. Schmitz, O. Pedersen, O. Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation ATB-BMPA Exofacial photolabelling Glucose transporter Skeletal muscle Subcellular fractionation 0014-5793 00145793 Elsevier |
| shingle_title_1 | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA |
| shingle_title_2 | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA |
| shingle_title_3 | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA |
| shingle_title_4 | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:23:01.274Z |
| titel | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA |
| titel_suche | Glut 4 content in the plasma membrane of rat skeletal muscle: comparative studies of the subcellular fractionation method and the exofacial photolabelling technique using ATB-BMPA Employing subcellular membrane fractionation methods it has been shown that insulin induces a 2-fold increase in the Glut 4 protein content in the plasma membrane of skeletal muscle from rats. Data based upon this technique are, however, impeded by poor plasma membrane recovery and cross-contamination with intracellular membrane vesicles. The present study was undertaken to compare the subcellular fractionation technique with the technique using [^3H]ATB-BMPA exofacial photolabelling and immunoprecipitation of Glut 4 on soleus muscles from 3-week-old Wistar rats. Maximal insulin stimulation resulted in a 6-fold increase in 3-O-methylglucose uptake, and studies based on the subcellular fractionation method showed a 2-fold increase in Glut 4 content in the plasma membrane, whereas the exofacial photolabelling demonstrated a 6- to 7-fold rise in cell surface associated Glut 4 protein. Glucose transport activity was positively correlated with cell surface Glut 4 content as estimated by exofacial labelling. In conclusion: (1) the increase in glucose uptake in muscle after insulin exposure is caused by an augmented concentration of Glut 4 protein on the cell surface membrane, (2) at maximal insulin stimulation (20 mUml) approximately 40% of the muscle cell content of Glut 4 is at the cell surface, and (3) the exofacial labelling technique is more sensitive than the subcellular fractionation technique in measuring the amount of glucose transporters on muscle cell surface. |
| topic | W V U |
| uid | nat_lic_papers_NLZ173344356 |