An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate
Thangada, S. ; Alvares, K. ; Mangino, M. ; Usman, M.I. ; Rao, M.S. ; Reddy, J.K.
Amsterdam : Elsevier
Amsterdam : Elsevier
| ISSN: |
0014-5793
|
|---|---|
| Keywords: |
(Rat hepatocyte) ; Peroxisome proliferation ; Tissue culture ; mRNA
|
| Source: |
Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
|
| Topics: |
Biology
Chemistry and Pharmacology
Physics
|
| Type of Medium: |
Electronic Resource
|
| URL: |
| _version_ | 1798290811117371395 |
|---|---|
| autor | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| autorsonst | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| book_url | http://linkinghub.elsevier.com/retrieve/pii/0014-5793(89)80721-5 |
| datenlieferant | nat_lic_papers |
| fussnote | Using the normal adult rat hepatocytes, plated on rat tail collagen-coated dishes and fed a chemically defined medium, we demonstrate here that ciprofibrate at 0.1 mM concentration, increases significantly the mRNA levels of fatty acyl-CoA oxidase, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein, and thiolase (the three enzymes of the β-oxidation system), and causes peroxisome proliferation. Increase in mRNA levels of these genes was evident within 1 h and was maximal 24 h after the addition of ciprofibrate. In hepatocytes cultured in the absence of ciprofibrate, the basal levels of these enzymes were low and further declined with time. Concomitant treatment of hepatocytes with cycloheximide did not inhibit or superinduce the mRNA levels, indicating that this induction may represent a primary (direct) effect of this compound on the expression of these genes and does not apparently involve short-lived repressor protein(s). |
| hauptsatz | hsatz_simple |
| identnr | NLZ173150292 |
| issn | 0014-5793 |
| journal_name | FEBS Letters |
| materialart | 1 |
| package_name | Elsevier |
| publikationsort | Amsterdam |
| publisher | Elsevier |
| reference | 250 (1989), S. 205-210 |
| schlagwort | (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA |
| search_space | articles |
| shingle_author_1 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| shingle_author_2 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| shingle_author_3 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| shingle_author_4 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. |
| shingle_catch_all_1 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA 0014-5793 00145793 Elsevier |
| shingle_catch_all_2 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA 0014-5793 00145793 Elsevier |
| shingle_catch_all_3 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA 0014-5793 00145793 Elsevier |
| shingle_catch_all_4 | Thangada, S. Alvares, K. Mangino, M. Usman, M.I. Rao, M.S. Reddy, J.K. An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA (Rat hepatocyte) Peroxisome proliferation Tissue culture mRNA 0014-5793 00145793 Elsevier |
| shingle_title_1 | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate |
| shingle_title_2 | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate |
| shingle_title_3 | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate |
| shingle_title_4 | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Elsevier Journal Backfiles on ScienceDirect 1907 - 2002 |
| timestamp | 2024-05-06T08:22:39.882Z |
| titel | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate |
| titel_suche | An in vitro demonstration of peroxisome proliferation and increase in peroxisomal β-oxidation system mRNAs in cultured rat hepatocytes treated with ciprofibrate Using the normal adult rat hepatocytes, plated on rat tail collagen-coated dishes and fed a chemically defined medium, we demonstrate here that ciprofibrate at 0.1 mM concentration, increases significantly the mRNA levels of fatty acyl-CoA oxidase, enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein, and thiolase (the three enzymes of the β-oxidation system), and causes peroxisome proliferation. Increase in mRNA levels of these genes was evident within 1 h and was maximal 24 h after the addition of ciprofibrate. In hepatocytes cultured in the absence of ciprofibrate, the basal levels of these enzymes were low and further declined with time. Concomitant treatment of hepatocytes with cycloheximide did not inhibit or superinduce the mRNA levels, indicating that this induction may represent a primary (direct) effect of this compound on the expression of these genes and does not apparently involve short-lived repressor protein(s). |
| topic | W V U |
| uid | nat_lic_papers_NLZ173150292 |