Hydrolysis of microcystins and nodularin by microwave radiation
| ISSN: |
1612-1112
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|---|---|
| Keywords: |
Column liquid chromatography ; Microwave hydrolysis ; Microcystin ; Nodularin ; D- and L-selective amino acid determination
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Chemistry and Pharmacology
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| Notes: |
Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798297327971074049 |
|---|---|
| autor | Reichelt, M. Hummert, C. Luckas, B. |
| autorsonst | Reichelt, M. Hummert, C. Luckas, B. |
| book_url | http://dx.doi.org/10.1007/BF02466910 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM209137738 |
| issn | 1612-1112 |
| journal_name | Chromatographia |
| materialart | 1 |
| notes | Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine. |
| package_name | Springer |
| publikationsjahr_anzeige | 1999 |
| publikationsjahr_facette | 1999 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1999 |
| publisher | Springer |
| reference | 49 (1999), S. 671-677 |
| schlagwort | Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination |
| search_space | articles |
| shingle_author_1 | Reichelt, M. Hummert, C. Luckas, B. |
| shingle_author_2 | Reichelt, M. Hummert, C. Luckas, B. |
| shingle_author_3 | Reichelt, M. Hummert, C. Luckas, B. |
| shingle_author_4 | Reichelt, M. Hummert, C. Luckas, B. |
| shingle_catch_all_1 | Reichelt, M. Hummert, C. Luckas, B. Hydrolysis of microcystins and nodularin by microwave radiation Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine. 1612-1112 16121112 Springer |
| shingle_catch_all_2 | Reichelt, M. Hummert, C. Luckas, B. Hydrolysis of microcystins and nodularin by microwave radiation Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine. 1612-1112 16121112 Springer |
| shingle_catch_all_3 | Reichelt, M. Hummert, C. Luckas, B. Hydrolysis of microcystins and nodularin by microwave radiation Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine. 1612-1112 16121112 Springer |
| shingle_catch_all_4 | Reichelt, M. Hummert, C. Luckas, B. Hydrolysis of microcystins and nodularin by microwave radiation Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Column liquid chromatography Microwave hydrolysis Microcystin Nodularin D- and L-selective amino acid determination Summary Microwave radiation has been successfully used for hydrolysis of the hepatotoxic cyclic peptides microcystins and nodularin. Set-up of the microwave device and the operating conditions for microwave hydrolysis were optimized. Results of the microwave hydrolysis were compared with results from conventional hydrolysis for 24 h at 110°C. Microwave hydrolysis of microcystins and nodularin for as little as 10 min at 160°C results in complete cleavage of peptide bonds and high recoveries of amino acids. Enantioselective determination of amino acids was achieved by use of a previously described HPLC method after pre-column derivatization withortho-phthaldialdehyde and the chiral thiolN-iso-butyryl-cysteine. 1612-1112 16121112 Springer |
| shingle_title_1 | Hydrolysis of microcystins and nodularin by microwave radiation |
| shingle_title_2 | Hydrolysis of microcystins and nodularin by microwave radiation |
| shingle_title_3 | Hydrolysis of microcystins and nodularin by microwave radiation |
| shingle_title_4 | Hydrolysis of microcystins and nodularin by microwave radiation |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T10:06:14.466Z |
| titel | Hydrolysis of microcystins and nodularin by microwave radiation |
| titel_suche | Hydrolysis of microcystins and nodularin by microwave radiation |
| topic | V |
| uid | nat_lic_papers_NLM209137738 |