Biological generation of tritiated vitamin D3 metabolites and their purification by high-performance liquid chromatography
Clemens, T. L. ; Fraher, L. J. ; O'Riordan, J. L. H. ; Little, C. J. ; Dale, A.
Springer
Published 1980
Springer
Published 1980
| ISSN: |
1612-1112
|
|---|---|
| Keywords: |
Tritiated dihydroxycholecalciferol metabolites ; Renal hydroxylase enzymes ; Sephadex LH 20 ; High-performance liquid chromatography
|
| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Chemistry and Pharmacology
|
| Notes: |
Summary A simple, reproducible method for the biological synthesis of tritiated 24,25-dihydroxycholecalciferol, 25,26-dihydroxycholecalciferol and 1,25-dihydroxycholecalciferol is described. Kidney homogenates from both vitamin D deficient and replete chicks were usedin vitro to generate these dihydroxylated metabolites using 25 (23,24-3H) hydroxycholecalciferol as the substrate. Tritiated products were purified by Sephadex LH 20 chromatography followed by high-performance liquid chromatography; the identity of each metabolite was established by chromatography with authentic crystalline preparations.
|
| Type of Medium: |
Electronic Resource
|
| URL: |