Fluorescence microscopic study of the formation of giant mitochondrial nuclei in the young ovules ofPelargonium zonale

Kuroiwa, T. ; Kuroiwa, H. ; Mita, T. ; Fujie, M.
Springer
Published 1990
ISSN:
1615-6102
Keywords:
Pelargonium zonale ; Ovale ; Giant mitochondrial nuclei ; DAPI ; Fluorescence microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The size of mitochondrial genomes in higher plants are known to range from 200 to 2400 kilobase pairs. However, we failed to identify cytochemically any mitochondria that contain an identifiable master mitochondrial genome. In the present experiments, we have found the giant mitochondrial nuclei which have the capacity for including the master mitochondrial genome in the young ovaries ofPelargonium zonale by use of a 4′-6-diamidino-2-phenylindole (DAPI) epifluorescence microscopy, a Technovit embedding, and a video-intensified photon counting system.
Type of Medium:
Electronic Resource
URL: