Cosmos pollen ontogeny: A scanning electron microscope study

Blackmore, S. ; Barnes, Susan H.
Springer
Published 1985
ISSN:
1615-6102
Keywords:
Compositae ; Cosmos bipinnatus ; Freeze-fracture ; Cytoplasmic maceration ; Pollen ontogeny ; Scanning electron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
Type of Medium:
Electronic Resource
URL:
_version_ 1798297349824446465
autor Blackmore, S.
Barnes, Susan H.
autorsonst Blackmore, S.
Barnes, Susan H.
book_url http://dx.doi.org/10.1007/BF01287676
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM208443835
issn 1615-6102
journal_name Protoplasma
materialart 1
notes Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
package_name Springer
publikationsjahr_anzeige 1985
publikationsjahr_facette 1985
publikationsjahr_intervall 8014:1985-1989
publikationsjahr_sort 1985
publisher Springer
reference 126 (1985), S. 91-99
schlagwort Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
search_space articles
shingle_author_1 Blackmore, S.
Barnes, Susan H.
shingle_author_2 Blackmore, S.
Barnes, Susan H.
shingle_author_3 Blackmore, S.
Barnes, Susan H.
shingle_author_4 Blackmore, S.
Barnes, Susan H.
shingle_catch_all_1 Blackmore, S.
Barnes, Susan H.
Cosmos pollen ontogeny: A scanning electron microscope study
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
1615-6102
16156102
Springer
shingle_catch_all_2 Blackmore, S.
Barnes, Susan H.
Cosmos pollen ontogeny: A scanning electron microscope study
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
1615-6102
16156102
Springer
shingle_catch_all_3 Blackmore, S.
Barnes, Susan H.
Cosmos pollen ontogeny: A scanning electron microscope study
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
1615-6102
16156102
Springer
shingle_catch_all_4 Blackmore, S.
Barnes, Susan H.
Cosmos pollen ontogeny: A scanning electron microscope study
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Compositae
Cosmos bipinnatus
Freeze-fracture
Cytoplasmic maceration
Pollen ontogeny
Scanning electron microscopy
Summary Ontogenetic data concerning pollen not only clarifies the mode of deposition of the elaborate walls but has considerable functional and taxonomic relevance. Hitherto such studies have used optical or transmission electron microscopy but here a recently devised preparative technique has enabled pollen development inCosmos bipinnatus to be studied using the scanning electron microscope. The technique involves freeze-fracturing of osmium fixed, cryoprotected anthers, maceration in dilute osmium tetroxide, critical point drying, sputter coating and examination. The processes of pollen wall development can then be observed in three dimensions, an important aid to understanding the spatial relationships involved in the determination of ornamentation and apertures. Details of the pollen and tapetum are described at various stages between meiosis and anthesis. A close conformity is demonstrated between the results obtained and those of earlier transmission electron microscopic studies of the same and related species although very different interpretations are made.
1615-6102
16156102
Springer
shingle_title_1 Cosmos pollen ontogeny: A scanning electron microscope study
shingle_title_2 Cosmos pollen ontogeny: A scanning electron microscope study
shingle_title_3 Cosmos pollen ontogeny: A scanning electron microscope study
shingle_title_4 Cosmos pollen ontogeny: A scanning electron microscope study
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source_archive Springer Online Journal Archives 1860-2000
timestamp 2024-05-06T10:06:35.698Z
titel Cosmos pollen ontogeny: A scanning electron microscope study
titel_suche Cosmos pollen ontogeny: A scanning electron microscope study
topic W
uid nat_lic_papers_NLM208443835