Properties of an RNA polymerase complex detected in influenza virus-infected cells
| ISSN: |
1432-8798
|
|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
|
| Topics: |
Medicine
|
| Notes: |
Summary Two RNA-dependent RNA polymerase activities are detected in the cytoplasm of influenza Ao/NWS infected cells; one activity has properties similar to the RNA-dependent RNA polymerase activity associated with the influenza Ao/NWS virion and is stimulated by Mn2+ at high ionic strength. The other activity is stimulated by Mg2+ at low ionic strength. Both cellular activities are associated with a heavy complex which sediments heterogeneously in the region of monosomes in a sucrose gradient; this polymerase complex is not bound to ribosomes. The polymerase complex contains one main protein which corresponds to the nucleocapsid protein (NP) of the virus; it also contains segmented viral RNA. The polymerase complex and the ribonucleoprotein of the virus have similar components and sedimentation properties.
|
| Type of Medium: |
Electronic Resource
|
| URL: |