Epitope mapping of the influenza A virus RNA polymerase PA using monoclonal antibodies
Hatta, M. ; Asano, Y. ; Masunaga, K. ; Ito, T. ; Okazaki, K. ; Toyoda, T. ; Kawaoka, Y. ; Ishihama, A. ; Kida, H.
Springer
Published 2000
Springer
Published 2000
ISSN: |
1432-8798
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Source: |
Springer Online Journal Archives 1860-2000
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Topics: |
Medicine
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Notes: |
Summary. βTo obtain reagents to functionally map the PA protein, we produced monoclonal antibodies specific to this protein. Twenty-two monoclonal antibodies reacting with PA protein in ELISA were divided into 10 groups on the basis of competitive binding patterns to this protein. Of these, seventeen monoclonal antibodies bound to PA polypeptide spanning amino acids 101β400 and three bound to that of amino acids 518β600, while the other two did not react with any PA polypeptides tested with the exception of the intact PA. Among these monoclonal antibodies, only five reacted with PA in A/PR/8/34 virus-infected cells in indirect immunofluorescence assay. Thus, we obtained monoclonal antibodies that recognize at least 10 distinct regions of the PA molecule. These monoclonal antibodies should be useful in dissecting functions of the PA protein.
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Type of Medium: |
Electronic Resource
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URL: |