Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses
| ISSN: |
1432-8798
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296015755804673 |
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| autor | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| autorsonst | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| book_url | http://dx.doi.org/10.1007/BF01310579 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM208226443 |
| issn | 1432-8798 |
| journal_name | Archives of virology |
| materialart | 1 |
| notes | Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9. |
| package_name | Springer |
| publikationsjahr_anzeige | 1994 |
| publikationsjahr_facette | 1994 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1994 |
| publisher | Springer |
| reference | 134 (1994), S. 421-426 |
| search_space | articles |
| shingle_author_1 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| shingle_author_2 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| shingle_author_3 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| shingle_author_4 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. |
| shingle_catch_all_1 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9. 1432-8798 14328798 Springer |
| shingle_catch_all_2 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9. 1432-8798 14328798 Springer |
| shingle_catch_all_3 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9. 1432-8798 14328798 Springer |
| shingle_catch_all_4 | Zhang, X. Herbst, W. Kousoulas, K. G. Storz, J. Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses Summary The nucleotide sequence of the S gene of the bovine respiratory coronavirus (BRCV) strain G95, which was isolated from nasal swabs of a calf suffering from respiratory disorders, was determined and compared with the S gene of the enteropathogenic bovine coronavirus (BECV) strain LY138. Sequence analysis revealed 98.7% nucleotide and 98.3% deduced amino acid identities between the S genes of BRCV-G95 and BECV-LY138 without any deletions or insertions. Nucleotide substitutions were distributed randomly throughout the gene. Five monoclonal antibodies specific for the S protein distinguished BRCV-G95 from BECV-L9, but failed to differentiate it from BECV-LY138 in Western blots under denatured and native conditions. BRCV-G95 induced cytopathic changes in cell cultures that were similar to BECV-LY138 but different from BECV-L9. These results suggest that strain BRCV-G95 is more closely related to the virulent strain BECV-LY138 than to the avirulent, cell culture-adapted strain BECV-L9. 1432-8798 14328798 Springer |
| shingle_title_1 | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| shingle_title_2 | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| shingle_title_3 | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| shingle_title_4 | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:45:22.819Z |
| titel | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| titel_suche | Comparison of the S genes and the biological properties of respiratory and enteropathogenic bovine coronaviruses |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM208226443 |