Mapping of the SLA complex class III region by pulsed field gel electrophoresis
| ISSN: |
1432-1777
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798297661194895360 |
|---|---|
| autor | Wu, L. Rothschild, M. F. Warner, C. M. |
| autorsonst | Wu, L. Rothschild, M. F. Warner, C. M. |
| book_url | http://dx.doi.org/10.1007/BF00352366 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM207331405 |
| issn | 1432-1777 |
| journal_name | Mammalian genome |
| materialart | 1 |
| notes | Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species. |
| package_name | Springer |
| publikationsjahr_anzeige | 1995 |
| publikationsjahr_facette | 1995 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1995 |
| publisher | Springer |
| reference | 6 (1995), S. 607-610 |
| search_space | articles |
| shingle_author_1 | Wu, L. Rothschild, M. F. Warner, C. M. |
| shingle_author_2 | Wu, L. Rothschild, M. F. Warner, C. M. |
| shingle_author_3 | Wu, L. Rothschild, M. F. Warner, C. M. |
| shingle_author_4 | Wu, L. Rothschild, M. F. Warner, C. M. |
| shingle_catch_all_1 | Wu, L. Rothschild, M. F. Warner, C. M. Mapping of the SLA complex class III region by pulsed field gel electrophoresis Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species. 1432-1777 14321777 Springer |
| shingle_catch_all_2 | Wu, L. Rothschild, M. F. Warner, C. M. Mapping of the SLA complex class III region by pulsed field gel electrophoresis Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species. 1432-1777 14321777 Springer |
| shingle_catch_all_3 | Wu, L. Rothschild, M. F. Warner, C. M. Mapping of the SLA complex class III region by pulsed field gel electrophoresis Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species. 1432-1777 14321777 Springer |
| shingle_catch_all_4 | Wu, L. Rothschild, M. F. Warner, C. M. Mapping of the SLA complex class III region by pulsed field gel electrophoresis Abstract The fine order of genes in the class III region of the swine major histocompatibility complex (MHC), the SLA complex, was examined by pulsed field gel electrophoresis (PFGE) and Southern blot analysis. Four genes, C2, HSP70, TNFα, and CYP21, were analyzed. The CYP21, C2, and HSP70 genes were all located within a 200-kb NotI fragment. The C2, HSP70, and TNFα genes cohybridized to a 420-kb SalI fragment. The TNFα gene is linked to the class I region by a 390-kb NotI fragment. Combined with a previous study from our lab, the order of genes in the SLA complex is class II-class III [(CYP21/C4)-(Bf/C2/HSP70)-TNFα]-class I. The size of the class III region from CYP21 to TNFα is estimated to be 500 kb. This size and the order of the genes in the swine class III region are similar to those of human, mouse, goat, and rabbit, which confirms the high conservation of class III gene organization across species. 1432-1777 14321777 Springer |
| shingle_title_1 | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| shingle_title_2 | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| shingle_title_3 | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| shingle_title_4 | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T10:11:32.252Z |
| titel | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| titel_suche | Mapping of the SLA complex class III region by pulsed field gel electrophoresis |
| topic | W WW-YZ |
| uid | nat_lic_papers_NLM207331405 |