Helicobacter pylori in a costa rican dyspeptic patient population
| ISSN: |
1435-4373
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798296203573592064 |
|---|---|
| autor | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| autorsonst | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| book_url | http://dx.doi.org/10.1007/BF01974546 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM206281099 |
| issn | 1435-4373 |
| journal_name | European journal of clinical microbiology & infectious diseases |
| materialart | 1 |
| notes | Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material. |
| package_name | Springer |
| publikationsjahr_anzeige | 1994 |
| publikationsjahr_facette | 1994 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1994 |
| publisher | Springer |
| reference | 13 (1994), S. 253-257 |
| search_space | articles |
| shingle_author_1 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| shingle_author_2 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| shingle_author_3 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| shingle_author_4 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. |
| shingle_catch_all_1 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. Helicobacter pylori in a costa rican dyspeptic patient population Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material. 1435-4373 14354373 Springer |
| shingle_catch_all_2 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. Helicobacter pylori in a costa rican dyspeptic patient population Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material. 1435-4373 14354373 Springer |
| shingle_catch_all_3 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. Helicobacter pylori in a costa rican dyspeptic patient population Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material. 1435-4373 14354373 Springer |
| shingle_catch_all_4 | Morera-Brenes, B. Sierra, R. Barrantes, R. Jonasson, J. Nord, C. E. Helicobacter pylori in a costa rican dyspeptic patient population Abstract Gastric biopsies from 65 Costa Rican dyspeptic patients were investigated for the presence ofHelicobacter pylori DNA by polymerase chain reaction (PCR) amplification of 16S rRNA sequences. Both frozen and paraffin-embedded samples were used, and the results were compared with bacterial culture and histological examination.Helicobacter pylori DNA was detected by PCR in 60 (92 %) of the patients, andHelicobacter pylori strains confirmed by PCR could be isolated from 37 of them. Altogether, 59 patients were shown to be infected using the combination of culture and histology as the reference method. The sensitivity of PCR analysis of frozen material was 98 % (58/59). The PCR analysis of paraffin-embedded samples seemed less reliable than that of frozen biopsy material. 1435-4373 14354373 Springer |
| shingle_title_1 | Helicobacter pylori in a costa rican dyspeptic patient population |
| shingle_title_2 | Helicobacter pylori in a costa rican dyspeptic patient population |
| shingle_title_3 | Helicobacter pylori in a costa rican dyspeptic patient population |
| shingle_title_4 | Helicobacter pylori in a costa rican dyspeptic patient population |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:48:22.424Z |
| titel | Helicobacter pylori in a costa rican dyspeptic patient population |
| titel_suche | Helicobacter pylori in a costa rican dyspeptic patient population |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM206281099 |