Correlation between intestinal immune response to colonization factor antigen/I and acquired resistance to enterotoxigenicEscherichia coli diarrhea in an adult rabbit model
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1435-4373
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Springer Online Journal Archives 1860-2000
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Topics: |
Medicine
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Abstract Immunoprotection against diarrhea caused by colonization factor antigen/I (CFA/I)-positive, human-associated, enterotoxigenicEscherichia coli was investigated using the adult rabbit intestinal temporary ligation technique. An oral dose of 1×108 viable cells of enterotoxigenicEscherichia coli strain H-10407 (078:H11:CFA/I) produced diarrhea in all animals challenged. Rabbits allowed to survive this challenge dose were re-challenged approximately six weeks later with the result that four of seven (57 %) did not develop diarrhea. Peroral immunization of rabbits with purified CFA/I elicited protection against challenge with strain H-10407; this protection was dose-related and CFA/I-specific. Immunoprotection did not correlate with a systemic antibody response. CFA/I produced a relatively poor immune response in terms of the number of IgM- and IgG-producing cells in the lamina propria of the animals but did elicit a vigorous increase in the number of intestinal IgA- and anti-CFA/I-producing cells. There was a highly significant inverse relationship between the number of IgA- and anti-CFA/I-producing cells in the lamina propria of the rabbits and the diarrhea response to the challenge strain H-10407 (correlation coefficients of −0.616 and −0.678 respectively). It is concluded that anti-CFA/I antibody, probably of the IgA class, is the major immune response to orally administered CFA/I and that this response is highly immunoprotective.
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Electronic Resource
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