High throughput analysis of 10 microsatellite and 11 diallelic polymorphisms on the human Y-chromosome

Thomas, M.G. ; Bradman, N. ; Flinn, H.M.
Springer
Published 1999
ISSN:
1432-1203
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Medicine
Notes:
Abstract. We describe an integrated approach to the determination of complex Y chromosome haplotypes that is both fast and relatively inexpensive. The method employs GeneScan technology to enable a researcher to assay repeat number variation at ten microsatellite loci and determine the status of 11 diallelic polymorphisms. The method requires only four PCRs and four GeneScan runs per sample and is relatively insensitive to sample DNA concentration.
Type of Medium:
Electronic Resource
URL: