Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H
ISSN: |
1617-4623
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Keywords: |
Key words Barley ; Physical mapping ; Recombination ; Powdery mildew
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Source: |
Springer Online Journal Archives 1860-2000
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Topics: |
Biology
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Notes: |
Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval.
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Type of Medium: |
Electronic Resource
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URL: |
_version_ | 1798297393131683840 |
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autor | DeScenzo, Richard A. Wise, R. P. |
autorsonst | DeScenzo, Richard A. Wise, R. P. |
book_url | http://dx.doi.org/10.1007/BF02172376 |
datenlieferant | nat_lic_papers |
hauptsatz | hsatz_simple |
identnr | NLM205529003 |
issn | 1617-4623 |
journal_name | Molecular genetics and genomics |
materialart | 1 |
notes | Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval. |
package_name | Springer |
publikationsjahr_anzeige | 1996 |
publikationsjahr_facette | 1996 |
publikationsjahr_intervall | 8004:1995-1999 |
publikationsjahr_sort | 1996 |
publisher | Springer |
reference | 251 (1996), S. 472-482 |
schlagwort | Key words Barley Physical mapping Recombination Powdery mildew |
search_space | articles |
shingle_author_1 | DeScenzo, Richard A. Wise, R. P. |
shingle_author_2 | DeScenzo, Richard A. Wise, R. P. |
shingle_author_3 | DeScenzo, Richard A. Wise, R. P. |
shingle_author_4 | DeScenzo, Richard A. Wise, R. P. |
shingle_catch_all_1 | DeScenzo, Richard A. Wise, R. P. Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H Key words Barley Physical mapping Recombination Powdery mildew Key words Barley Physical mapping Recombination Powdery mildew Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval. 1617-4623 16174623 Springer |
shingle_catch_all_2 | DeScenzo, Richard A. Wise, R. P. Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H Key words Barley Physical mapping Recombination Powdery mildew Key words Barley Physical mapping Recombination Powdery mildew Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval. 1617-4623 16174623 Springer |
shingle_catch_all_3 | DeScenzo, Richard A. Wise, R. P. Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H Key words Barley Physical mapping Recombination Powdery mildew Key words Barley Physical mapping Recombination Powdery mildew Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval. 1617-4623 16174623 Springer |
shingle_catch_all_4 | DeScenzo, Richard A. Wise, R. P. Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H Key words Barley Physical mapping Recombination Powdery mildew Key words Barley Physical mapping Recombination Powdery mildew Abstract Variants of the pulsed-field gel electrophoresis technique were used in conjunction with two-dimensional DNA gel electrophoresis (2-DDGE) to determine the ratio of physical to genetic distance in two genetically defined intervals on barley chromosome 1H. 2-DDGE analysis demonstrated that two loci that define a 0.3 cM interval, as determined by hybridization with BCD249, reside on a single 450-kb MluI fragment. This result indicates a maximum ratio of physical to genetic distance in this interval of 1500 kb/cM as compared to 3.7–4.2 Mb/cM for the barley genome as a whole. High molecular weight (HMW) DNA restricted with NotI and probed sequentially with MWG068 and BCD249 yield diffuse bands at approximately 2.8 Mb and 3.0 Mb in the C.I. 16151 and C.I. 16155 parental lines, respectively. These results suggest the maximum ratio of physical to genetic distance in the interval defined by these probes is 7.8 Mb/cM. Unique HMW DNA restriction fragment length polymorphisms (RFLP) were attributed to the presence of recombination breakpoints. Data from the recombination breakpoint analysis were used to estimate a ratio of physical to genetic distance of 2.5 Mb/cM in the Xbcd249.2-Xmwg068 interval and 0.465 Mb/cM in the Xbcd249.1-Xbcd249.2 interval. Both physical linkage and recombination breakpoint analysis indicate the Xbcd249.1-Xbcd249.2 interval is approximately five-fold smaller, physically, than the Xbcd249.2-Xmwg068 interval. 1617-4623 16174623 Springer |
shingle_title_1 | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
shingle_title_2 | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
shingle_title_3 | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
shingle_title_4 | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
source_archive | Springer Online Journal Archives 1860-2000 |
timestamp | 2024-05-06T10:07:15.805Z |
titel | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
titel_suche | Variation in the ratio of physical to genetic distance in intervals adjacent to the Mla locus on barley chromosome 1H |
topic | W |
uid | nat_lic_papers_NLM205529003 |