Complementation analysis of auxotrophic mutants of the moss, Physcomitrella patens, using protoplast fusion
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1617-4623
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Summary Fusion of protoplasts from the moss, Physcomitrella patens, was induced using polyethyleneglycol. Protoplasts were isolated from six nicotinic acid auxotrophic strains of independent origin and fusion was induced in all possible pairwise combinations. Complementation was detected by the ability to recover hybrids able to grow without nicotinic acid supplement. On the basis of the results presented, three nonoverlapping complementation groups were identified.
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Electronic Resource
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