Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing
Ibrahim, M. E. ; Hag-Ali, M. ; El-Hassan, A. M. ; Theander, T. G. ; Kharazmi, A.
Springer
Published 1994
Springer
Published 1994
| ISSN: |
1432-1955
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|---|---|
| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract A total of 17Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain ofL. major was adapted to grow at a high concentration of Pentostam (1000 μg/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20μg/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.
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| Type of Medium: |
Electronic Resource
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| URL: |