Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L.
| ISSN: |
1432-2048
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|---|---|
| Keywords: |
Caffeine ; Catabolism pathways ; Coffea ; Purine alkaloids
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
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| Notes: |
Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis.
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295918026424320 |
|---|---|
| autor | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| autorsonst | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| book_url | http://dx.doi.org/10.1007/BF00620048 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM204756499 |
| issn | 1432-2048 |
| journal_name | Planta |
| materialart | 1 |
| notes | Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis. |
| package_name | Springer |
| publikationsjahr_anzeige | 1996 |
| publikationsjahr_facette | 1996 |
| publikationsjahr_intervall | 8004:1995-1999 |
| publikationsjahr_sort | 1996 |
| publisher | Springer |
| reference | 198 (1996), S. 334-339 |
| schlagwort | Caffeine Catabolism pathways Coffea Purine alkaloids |
| search_space | articles |
| shingle_author_1 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| shingle_author_2 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| shingle_author_3 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| shingle_author_4 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. |
| shingle_catch_all_1 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. Caffeine Catabolism pathways Coffea Purine alkaloids Caffeine Catabolism pathways Coffea Purine alkaloids Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis. 1432-2048 14322048 Springer |
| shingle_catch_all_2 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. Caffeine Catabolism pathways Coffea Purine alkaloids Caffeine Catabolism pathways Coffea Purine alkaloids Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis. 1432-2048 14322048 Springer |
| shingle_catch_all_3 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. Caffeine Catabolism pathways Coffea Purine alkaloids Caffeine Catabolism pathways Coffea Purine alkaloids Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis. 1432-2048 14322048 Springer |
| shingle_catch_all_4 | Ashihara, H. Monteiro, A. M. Moritz, T. Gillies, F. M. Crozier, A. Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. Caffeine Catabolism pathways Coffea Purine alkaloids Caffeine Catabolism pathways Coffea Purine alkaloids Abstract In a study of purine alkaloid catabolism pathways in coffee,14C-labelled theobromine, caffeine, theophylline and xanthine were incubated with leaves ofCoffea arabica. Incorporation of label into14CO2 was determined and methanol-soluble metabolites were analysed by high-performance liquid chromatography-radiocounting. The data obtained demonstrate catabolism of caffeine → theophylline → 3-methylxanthine → xanthine. Xanthine is degraded further by the conventional purine catabolism pathway to CO2 and NH3 via uric acid, allantoin and allantoic acid. The conversion of caffeine to theophylline is the rate-limiting step in purine alkaloid catabolism and provides a ready explanation for the high concentration of endogenous caffeine found inC. arabica leaves. Although theobromine is converted primarily to caffeine, a small portion of the theobromine pool appears to be degraded to xanthine by a caffeine-independent pathway. In addition to being broken down to CO2, via the purine catabolism pathway, xanthine is metabolised to 7-methylxanthine. Metabolism of [2-14C]xanthine byC. arabica leaves in the presence of 5 mM allopurinol results in very large increases in incorporation of radioactivity into 7-methylxanthine as degradation of the substrate via the purine catabolism pathway is blocked. The identity of 7-methylxanthine in these studies was confirmed by gas chromatography-mass spectrometry analysis. 1432-2048 14322048 Springer |
| shingle_title_1 | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| shingle_title_2 | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| shingle_title_3 | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| shingle_title_4 | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:43:50.063Z |
| titel | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| titel_suche | Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabica L. |
| topic | W |
| uid | nat_lic_papers_NLM204756499 |