Immunohistochemistry of endogenous l-DOPA in the rat posterior hypothalamus

Tison, F. ; Mons, N. ; Geffard, M. ; Henry, P.
Springer
Published 1990
ISSN:
1432-119X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Medicine
Notes:
Summary The aim of this work was to study l-DOPA-containing neuronal structures of the rat posterior and dorsal hypothalamus by means of immunohistochemistry using antiserum against glutaraldehyde conjugated l-DOPA. Aspects and distribution of l-DOPA immunoreaction among cells of the supramammillary nucleus and the A11, A13c and A13 cell groups are described and compared to dopamine immunoreactivity, mainly through a double colored labelling procedure employing a color modification of the DAB reaction by metallic ions. Differences between l-DOPA and dopamine stainings within cell groups as the presence of cells with predominant or exclusive l-DOPA coloration are tentatively explained under the light of previous findings using immunohistochemistry of catecholamines synthesizing enzymes and catecholamines histofluorescence.
Type of Medium:
Electronic Resource
URL: