HSP70-2 is part of the synaptonemal complex in mouse and hamster spermatocytes
Allen, J. W. ; Dix, D. J. ; Collins, B. W. ; Merrick, B. A. ; He, C. ; Selkirk, J. K. ; Poorman-Allen, P. ; Dresser, M. E. ; Eddy, E. M.
Springer
Published 1996
Springer
Published 1996
| ISSN: |
1432-0886
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Biology
Medicine
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| Notes: |
Abstract Mouse spermatogenic cells are known to express HSP70-2, a member of the HSP70 family of heatshock proteins. The purpose of the present study was to characterize further the expression and localization of HSP70-2 in meiotic cells of mice and hamsters. After separating mouse spermatogenic cells into cytoplasmic and nuclear fractions, proteins were separated by two-dimensional gel electrophoresis and detected with HSP-specific antibodies. Of several HSP70 proteins identified in the cytoplasm, only HSC70 and HSP70-2 were also detected in the nucleus. Immunocytological analyses of spermatocyte prophase cells revealed that HSP70-2 was associated with the synaptonemal complex. Surfacespread synaptonemal complexes at pachytene and diplotene stages labeled distinctly with the antiserum to HSP70-2. Synaptonemal complexes from fetal mouse oocytes failed to show any evidence of HSP70-2. Reverse-transcriptase-polymerase chain reaction (RT-PCR) analyses of gene expression confirmed this sex specificity; Hsp70-2 mRNA was detected in mouse testes, but not ovaries. These findings are suggestive of a previously unsuspected sexual dimorphism in structure and/or function of the synaptonemal complex.
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| Type of Medium: |
Electronic Resource
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| URL: |