Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve
Tomei, G. ; Spagnoli, D. ; Ducati, A. ; Landi, A. ; Villani, R. ; Fumagalli, G. ; Sala, C. ; Gennarelli, T.
Springer
Published 1990
Springer
Published 1990
| ISSN: |
1432-0533
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|---|---|
| Keywords: |
Guinea pig ; Axonal injury ; Trauma ; Visual-evoked responses ; Horse radish peroxidase
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| Source: |
Springer Online Journal Archives 1860-2000
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| Topics: |
Medicine
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| Notes: |
Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI).
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| Type of Medium: |
Electronic Resource
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| URL: |
| _version_ | 1798295400007860224 |
|---|---|
| autor | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| autorsonst | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| book_url | http://dx.doi.org/10.1007/BF00294611 |
| datenlieferant | nat_lic_papers |
| hauptsatz | hsatz_simple |
| identnr | NLM203603931 |
| issn | 1432-0533 |
| journal_name | Acta neuropathologica |
| materialart | 1 |
| notes | Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI). |
| package_name | Springer |
| publikationsjahr_anzeige | 1990 |
| publikationsjahr_facette | 1990 |
| publikationsjahr_intervall | 8009:1990-1994 |
| publikationsjahr_sort | 1990 |
| publisher | Springer |
| reference | 80 (1990), S. 506-513 |
| schlagwort | Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase |
| search_space | articles |
| shingle_author_1 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| shingle_author_2 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| shingle_author_3 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| shingle_author_4 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. |
| shingle_catch_all_1 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI). 1432-0533 14320533 Springer |
| shingle_catch_all_2 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI). 1432-0533 14320533 Springer |
| shingle_catch_all_3 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI). 1432-0533 14320533 Springer |
| shingle_catch_all_4 | Tomei, G. Spagnoli, D. Ducati, A. Landi, A. Villani, R. Fumagalli, G. Sala, C. Gennarelli, T. Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Guinea pig Axonal injury Trauma Visual-evoked responses Horse radish peroxidase Summary A new model of focal axonal injury was reproduced by rapid and controlled elongation (uniaxial stretch) of the guinea pig optic nerve. Light microscopy study of optic nerve specimens after horseradish peroxidase injection into the vitreous of the animal's eye showed that axonal lesions were identical to those seen in human and primate post-traumatic diffuse axonal injury (DAI). The lesions were characterized by the formation of terminal clubs in severed axons and focal axonal enlargements in those axons that were lesioned-in-continuity. Visual-evoked potentials upon flash stimulation were recorded before and after injury. Mean amplitude and mean latency of occipital peaks were significantly elongated in the acute post-traumatic phase. Electron microscopy examination showed that the main axonal changes observed in this model were cytoskeleton disorganization, accumulation of axoplasm membrane-bound bodies at the site of terminal balls and dilatations-in-continuity and detachment of the axolemma from the myelin sheath. Such axonal alterations were similar to those found in many other biological models of central and peripheral axonal injuries in which the lesion was produced by invasive methods. This model is unique since it reproduces the same mechanism of injury and the identical lesions that have been demonstrated in humans and primates with post-traumatic (DAI). 1432-0533 14320533 Springer |
| shingle_title_1 | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| shingle_title_2 | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| shingle_title_3 | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| shingle_title_4 | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| sigel_instance_filter | dkfz geomar wilbert ipn albert fhp |
| source_archive | Springer Online Journal Archives 1860-2000 |
| timestamp | 2024-05-06T09:35:35.889Z |
| titel | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| titel_suche | Morphology and neurophysiology of focal axonal injury experimentally induced in the guinea pig optic nerve |
| topic | WW-YZ |
| uid | nat_lic_papers_NLM203603931 |