Electrophysiological analysis of fly retina

Hardie, R. C. ; Franceschini, N. ; McIntyre, P. D.
Springer
Published 1979
ISSN:
1432-1351
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Medicine
Notes:
Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
Type of Medium:
Electronic Resource
URL:
_version_ 1798295662938292224
autor Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
autorsonst Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
book_url http://dx.doi.org/10.1007/BF00663107
datenlieferant nat_lic_papers
hauptsatz hsatz_simple
identnr NLM20336046X
iqvoc_descriptor_title iqvoc_00000708:analysis
issn 1432-1351
journal_name Journal of comparative physiology
materialart 1
notes Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
package_name Springer
publikationsjahr_anzeige 1979
publikationsjahr_facette 1979
publikationsjahr_intervall 8024:1975-1979
publikationsjahr_sort 1979
publisher Springer
reference 133 (1979), S. 23-39
search_space articles
shingle_author_1 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
shingle_author_2 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
shingle_author_3 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
shingle_author_4 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
shingle_catch_all_1 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
Electrophysiological analysis of fly retina
Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
1432-1351
14321351
Springer
shingle_catch_all_2 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
Electrophysiological analysis of fly retina
Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
1432-1351
14321351
Springer
shingle_catch_all_3 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
Electrophysiological analysis of fly retina
Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
1432-1351
14321351
Springer
shingle_catch_all_4 Hardie, R. C.
Franceschini, N.
McIntyre, P. D.
Electrophysiological analysis of fly retina
Summary 1. Intracellular recordings have been made from over 100 central retinula cells (R7 and R8) in the fliesCalliphora stygia (wild type),Calliphora erythrocephala (chalky) andMusca domestica (white). 2. Spectral sensitivities determined with axial and non-axial illumination (Figs. 2, 3, 7, 9), polarisation sensitivities and their dependence upon stimulus wavelength (Table 2, Figs. 5, 6), and the effects of intense chromatic adaptation (Fig. 4) are described. 3. All retinula cells R7 investigated had a major peak of spectral sensitivity in the ultraviolet. One class with a peak at ca. 340 nm and less than 10% sensitivity remaining at wavelengths longer than 400 nm, are termed UV cells. Another class with a peak at ca. 360 nm and a long tail of sensitivity (〉10%) extending to 500 nm, are termed UT cells (Fig. 2). 4. The ratio of UT cells to UV cells was approximately 7∶3, this is similar to the ratio between the two classes of distal central rhabdomeres, seven yellow (7y) and seven pale (7p) (after Kirschfeld and Franceschini, 1977) (Table 1). 5. These results, in combination with recent microspectrophotometrical data (Kirschfeld et al., 1978; Kirschfeld, 1979) support the following interpretations: a) UV cells possess 7p rhabdomeres which contain an ultraviolet-absorbing rhodopsin that can be reversibly photoisomerised to a blue-absorbing metarhodopsin. b) UT cells possess 7y rhabdomeres and contain a different photopigment system to that found in UV (=7p) cells (Kirschfeld, 1979). Possibly this consists of a blue-absorbing rhodopsin and an ultraviolet sensitising pigment. The blue-absorbingphotostable pigment found in 7y rhabdomeres (Kirschfeld et al., 1978) has a screening function and modifies both spectral and polarisation sensitivity in UT cells. 6. The R8 cells encountered probably all had rhabdomeres lying beneath 7y rhabdomeres; their spectral sensitivity could be accurately modelled by the screening action of the pigments present in 7y rhabdomeres upon a visual pigment with a peak absorbance at ca. 520 nm (Fig. 9).
1432-1351
14321351
Springer
shingle_title_1 Electrophysiological analysis of fly retina
shingle_title_2 Electrophysiological analysis of fly retina
shingle_title_3 Electrophysiological analysis of fly retina
shingle_title_4 Electrophysiological analysis of fly retina
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timestamp 2024-05-06T09:39:46.552Z
titel Electrophysiological analysis of fly retina
titel_suche Electrophysiological analysis of fly retina
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